Abstract: Abstract: Objective To explore hepatic differentiation of human bone mesenchymal stem cell (BMSC) in 3-dimentional (3D) culture system of rat type-I collage and the polarity of differentiated hepatocyte. Methods BMSCs were isolated from adult bone marrow and differentiated to hepatocyte in 3D culture system of rat type-I collage. The morphological and structural characteristics of differentiated hepatocyte derived from BMSC in 3D culture were observed using HE staining. The expression of hepatocyte-specific markers of ALB and AFP were analyzed using immunohistochemical staining. The expression of hepatocyte polarity protein BSEP and SRB1 were assessed using the Immunofluorescence. Results Differentiated hepatocyte derived from BMSC formed tight junction and tubular or spherical structures in 3D culture system. The expression of ALB and AFP in hepatocytes differentiated from BMSC was positive. The expression of BSEP and SRBI in the 3D culture was positive and characteristic. Conclusion BMSC can differentiate to hepatocyte in 3D culture. The differentiated hepatocyte can form hepatocyte-specific polarity in 3D culture.

Key words: Bone mesenchymal stem cell, 3-dimentional culture, Hepatocyte, Polarity, Differentiation