Abstract: Objective Establish a database of ULBPs and MICA / B molecule express level in peripheral blood of sub-health state population, and provide a reference for evaluation the health of overall population and prevention of diseases. Methods The health statuses of volunteers were evaluated according to physical examination and questionnaire survey. After that, their serum protein levels of related molecules were detected by ELISA. Results The average concentrations of MICA, MICB, ULBP1 and ULBP2 in serum were higher in sub-health population compared with the healthy group. However, the levels of ULBP3, ULBP4 and ULBP5 were lower than those in healthy group. Univariate analysis showed that only MICB has significant difference (P <0.01) between the sub-healthy group and healthy group. Multivariate logistic regression analysis also confirmed this result. In addition, serum levels of MICA and ULBP3 in male was statistically significant higher than those in female (P<0.05). Additionally, ULBP2 and ULBP4 increase with age. Conclusions Together with other immune parameters, serum levels of soluble MICB may be useful in the diagnosis of sub-health.

Key words: Sub-health, ULBPs, MICA/B