Abstract: Abstract: Objective To explore the expression of DLL4 in A549 after CoCl2 treatment and elucidate the relationship between hypoxia induced factor-1α (HIF-1α) and delta like legend 4 (DLL4). Migration of endothelial cells in the condition of cancer culture supernatant was also observed. Methods Cell proliferation was detected by EdU incorporation assay. Expression of DLL4 and HIF-1α were detected by immunofluorescence staining and western-blot. Migration of endothelial cells was measured in a cell scratch wound model. Results The viability and proliferation of A549 were not significantly decreased after the CoCl2 (200 μmol/L) treatment. Expression of HIF-1α and DLL4 were increased in A549 after CoCl2 treatment compared to normal condition. Culture supernatant from CoCl2 treated A549 could enhance the migration of endothelial cells. Conclusion HIF-1α and DLL4 could be stimulated under hypoxic condition in A549. A549 in hypoxic condition can trigger the migration of endothelial cells, which is critical to angiogenesis. DLL4 might participate in tumor angiogenesis and it is a promising target in tumor treatment.

Key words: lung cancer, DLL4, migration of endothelial cell