Abstract: 【Abstract】 Aims: Continually to deeply explore BHLHB2 related molecular biology functions, which was proved a novel and independent prognostic marker in pancreatic ductal adenocarcinoma by our protophase experimental results. Methods: Knock-down of BHLHB2 in pancreatic cancer cell line-SU86.86 was achieved by siRNA treatment. Migration essay was performed to research the relationship between BHLHB2 and cell migration. The proliferation assay induced by ACT-D to explore BHLHB2 roles in cell apoptosis. Associativity between BHLHB2 and EMT was analyzed using immunoblot analysis. Results: BHLHB2 silencing with RNAi significantly promote cell migrate, and induced cell apoptosis by Actinomycin D (ACT-D) (10ng/ml, increased 1.32 fold, p<0.05; 100ng/ml, increased 1.41 fold, p<0.05). By Western Blot, BHLHB2 silencing significantly deduced E-cadherin expression (decreased 11.35 fold, p<0.01), which implicated BHLHB2 was correlated with epithelial mesenchymal transform (EMT). Conclusions: BHLHB2 has multiple biological functions, closely associated with cell migration, cell apoptosis and EMT in human pancreatic cancer.

Key words: Pancreatic cancer, Biological function, Apoptosis