Abstract: 【Abstract】Objective: To study the genotype distribution of a single nucleotide polymorphism(SNP) rs-2796516(G>A) in 5’-AMP-activated protein kinase(AMPK) alpha-2 subunit gene in gansu area. To investigate the association between AMPKα2 subunit gene polymorphism and type 2 diabetes mellitus(T2DM), insulin resistance(IR) and plasma resistin and adiponectin. Methods: In this study, 188 patientes with T2DM(case group) and 106 healthy persons(control group) from gansu area were enrolled. A SNP rs-2796516(G>A) in AMPKα2 subunit gene was genotyped in all the subjects enrolled using PCR and restriction fragment length polymorphism(RFLP). ELISA technique were employed to determine the plasma adiponectin and resistin concentration. At the same time the fasting blood glucose, insulin and blood lipid were detected. Results: (1)There was polymorphism of AMPK-rs2796516 in both T2DM and normal subjects. There was significant difference of genotype constituent ratio and A allele frequency in two groups. (2) In case group, AMPKα2 subunit gene polymorphism were significantly related to insulin resistance, blood lipid and the concentration of plasma adiponectin and resistin. Compared with GG and GA group, the concentration of serum TG, TCH, LDL, resistin were higher in AA group, the concentration of serum adiponectin was lower in AA group. Conclusion: The polymorphism of AMPK-rs2796516 G/A was revelant with T2DM. It was presumed that A allele maybe the risk gene of T2DM. There was significant association between the polymorphism of AMPK-rs2796516 and the concentration of plasma adiponectin and resistin. Adiponectin and resistin took part in the onset of IR and T2DM through AMPK pathways.

Key words: 5’-AMP-activated protein kinase(AMPK), type 2 diabetes mellitus (T2DM), resistin, adiponectin