Abstract: Abstract: Objective To investigate the effecti and mechanism of Noggin protein in proliferation and invasion of human breast cancer cell line MDA-MB-231. Methods MDA-MB-231 cells were infected with recombinant Noggin adenovirus. MTT, Wound-healing experiment and Matrigel invasion assays were performed to examine the change of proliferation, movement and invasion. The expression of MMP-1 and CXCR4 was evaluated by real-time PCR and Western blot. Results The growth of MDA-MB-231 cells infected with Ad-Noggin is faster than control group（P<0.05）, while scratch repair time extended. Transwell cell invasion assay shown that the invasive ability of MDA-MB-231 cells were inhibited in Noggin group(79±4), compared with GFP group(135±7)（P<0.05）. Using real-time PCR and western blot, the expression of CXCR4 and MMP1 was confirmed to decrease. Conclusion Noggin protein can enhance the proliferation of MDA-MB-231 cell line, and inhibit its invasion and metastases. Maybe the cancer-related gene CXCR4 and MMP1 was involved in it.

Key words: Key words: Noggin, breast cancer, osseous metastasis, MDA-MB-231