Abstract: Objective To investigate the effect of the activation of P38MAPK signaling pathway on the intracellular β-amyloid(Aβ) production in human neuroblastoma SH-SY5Y. Methods SH-SY5Y cells were used to test intracellular Aβ levels by ELISA after activation of P38MAPK signaling pathway by anisomycin for 1 and 72 h.The mRNA and protein levels of APP, PS1 and BACE1 were examined by real time quantitative PCR and Western blot respectively. Results The expression of Phospho-P38MAPK was increased after anisomycin treatment 1and 72 h; the production of Aβ1-40 was about (46.88±3.60) pg/mL[p<0.05,Con group was (39.09±1.60) pg/mL]after anisomycin treatment for 72h; APP and PS1 mRNA was increased after anisomycin treatment for 1h, while APP, PS1 and BACE1 mRNA was significantly increased after anisomycin treatment for 72h;the protein levels of APP, PS1 and BACE1 were increased as 1.45-fold,1.38-fold and 1.60-fold respectively as control after anisomycin treatment for 72h while nothing alter for 1h. Conclusion Activation of P38MAPK signaling pathway could increase Aβ production and play a role in the pathygensis of Alzheimer's disease(AD).

Key words: Alzheimer's disease, p38MAPK, β-Amyloid(Aβ).