Abstract: Objective To explore the effects of 3C protease on host cell proteins by proteomics techniques. Methods 293T cells were transfected with plasmid encoding EV71 3C, cell lysates were analyzed by using two-dimensional fluorescence difference gel electrophoresis (2D-DIGE) and mass spectrometry (MS) analysis. Down-regulated protein was confirmed by Western blot analysis. Results The expression of EV71 3C in 293T cells is confirmed. A total of 5 altered proteins were identified after 3C overexpression. Among them, expression of 4 proteins increased, while expression of 1 protein decreased. Down-regulated protein HSPA4 was confirmed by Western blot. HSPA4 expression was determined by performing densitometric analyses in HeLa cells. The results show that HSPA4 expression was 46.8%±10.1% (P＜0.05) compared to normal cells. Conclusions Overexpression of EV71 3C regulates the protein expression in 293T cells.

Key words: Enterovirus 71, 3C, proteomics differences