Abstract: Objective To identify the regulation of PPARγ and PGC1α on CIDEC transcription. And to determine the Function of CIDEC in adipose metabolism. Methods Reconstructing CIDEC promoter reporters including a series of 5’-deletions and cis-element mutations. Dual-luciferase assay was performed to screen PPARγ binding sites in HepG2 cells. Pioglitazone, which is the PPARγ agonist, was used in 3T3-L1 cells to detect the CIDEC mRNA level. Adenoviral CIDEC was constructed and over-expressed CIDEC gene through adenovirus in primary hepatocytes to examine the effect of CIDEC on liver adipose metabolism. Results PPARγ and PGC1α can stimulate CIDEC transcription obviously. Over-expressing CIDEC stimulate gene FAS 3.47±0.17 (p<0.05) and ACC 3.95±0.57 (p<0.05), and adipose synthesis in hepatocytes. Conclusion Transcription of CIDEC can be activated by PPARγ and its coactivitor PGC1α. This activation result in accumulation of fat in cells and liver.

Key words: PPARγ, PGC1α, CIDEC, transcriptional regulation