基础医学与临床 ›› 2008, Vol. 28 ›› Issue (10): 0-0.

• 研究论文 •    下一篇

互隔交链孢酚增加NIH3T3细胞中DNA聚合酶β表达

赵继敏 金戈 李沛 赵明耀 杨洪艳 郑智敏 董子明   

  1. 郑州大学基础医学院 郑州大学基础医学院
  • 收稿日期:2007-12-06 修回日期:2008-01-21 出版日期:2008-10-25 发布日期:2008-10-25
  • 通讯作者: 赵继敏

Alternariol enhanced DNA Polymerase β Expression in NIH3T3 Cells

Ji-min ZHAO, Ge JIN, Pei LI, Ming-yao ZHAO, Hong-yan YANG, Zhi-min ZHENG, Zi-ming DONG   

  1. Basic Medical College, Zhengzhou University
  • Received:2007-12-06 Revised:2008-01-21 Online:2008-10-25 Published:2008-10-25
  • Contact: Ji-min ZHAO,

摘要： 目的 研究互隔交链孢酚（AOH）对小鼠胚胎成纤维细胞NIH3T3中DNA聚合酶β（DNA polβ）表达的影响。方法 采用半定量RT-PCR、细胞免疫化学和Western blot方法检测细胞中DNA POLβmRNA和蛋白的表达。结果 与对照组相比，AOH可以引起NIH3T3细胞中DNAPOLβ基因的mRNA水平和蛋白水平表达增高（并呈一定的剂量效应关系）（P<0.05）。结论 AOH可以引起NIH3T3细胞中DNA POLβ基因表达增高，可能有助于细胞应对AOH引起的DNA损伤。

关键词: DNA聚合酶β, 互隔交链孢酚, NIH3T3细胞

Abstract: Objective To study the effects of Alternariol (AOH) on DNA polymerase β (DNA polβ)expression in NIH3T3 cells. Methords RT-PCR and Immunocytochemistry and Western blot were used to detected mRNA and the protein levels of DNA polβ in NIH3T3 cell line induced by AOH. Results The expression of DNA polβ in NIH3T3 cells contaminated by AOH was significantly higher than that in the control group(P<0.05). Conclusion AOH induces overexpression of DNA polβ in the NIH3T3 Cells, which could be responsible to the DNA damage in cells caused by AOH.

Key words: DNA polβ, alternariol, NIH3T3 cells


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