移植MMSCs改善大鼠实验性心肌梗死后心室重构

基础医学与临床 ›› 2010, Vol. 30 ›› Issue (1): 48-53.

移植MMSCs改善大鼠实验性心肌梗死后心室重构

伍徐娴何志旭沈祥春周中军刘鉴

贵阳医学院贵阳医学院

收稿日期:2009-02-11 修回日期:2009-05-19 出版日期:2010-01-05 发布日期:2010-01-05
通讯作者: 何志旭

Transplanting Marrow Mesenchymal Stem Cells can Improve the Ventricular Remodeling and Heart Functions to Acute MI Rat Models

Xu-xian WU, Zhi-xu HE, Xiang-chun SHEN, Zhong-jun ZHOU, Jian LIU

Guiyang Medical College Guiyang Medical College

Received:2009-02-11 Revised:2009-05-19 Online:2010-01-05 Published:2010-01-05
Contact: Zhi-xu HE,

摘要/Abstract

摘要： 目的探讨骨髓间充质干细胞（MMSCs）移植对大鼠实验性心肌梗死（MI）后心功能及心室重构的影响。方法开胸结扎冠状动脉左前降支（LAD）制作MI动物模型，造模成功后12～14d经舌下静脉移植已转染增强型绿色荧光蛋白（pEGFP-C1）的MMSCs，分4及8周检测动物的血流动力学、左心及全心质量指数、微血管密度测定、双盲计数法测定胶原含量，荧光显微镜观察绿色荧光的表达。结果移植组心率（HR）较同期模型组显著下降，4周时血压无明显改变；8周时则显著增高。移植组左心质量指数和全心质量指数均较同期模型组显著降低。移植组的微血管密度较同期模型组明显增高。移植组的胶原含量较同期模型组显著降低。结论移植MMSCs可改善实验性MI大鼠心功能及心室重构。

关键词: 心肌梗死, 骨髓间充质干细胞, 心室重构, 增强型绿色荧光蛋白

Abstract: Objective To study the effects of marrow mesenchymal stem cells on heart functions and ventricular remodeling after myocardial infarction in rats. Methods Myocardial infarction was operated by ligation of the left anterior descending coronary artery ( LAD ) in adult SD rats. 4 and 8 weeks after MMSCs implantation, hemodynamic evaluations, left ventricular weight/body weight ratio and heart weight/body weight ratio were determined. HE staining was performed for double-blind counting the density of microvasculars and Van Gieson staining was used for measurements and calculation of the myocardial fibrillar collagen. Then we investigated the migration and evolution of MSCs in vivo by fluorescent microscope. Results First of all, HR were significantly decreased in transplantation MMSCs group. After transplantation 8 weeks, bodies weight in transplantation MMSCs group reached to control group's. At the same time, SBP, DBP and MBP, were significantly increased in transplantation MMSCs group. HR were significantly decreased in transplantation MMSCs group. Secondly, left ventricular weight/body weight ratio and heart weight/body weight ratio were significantly decreased after transplantation MMSCs 4 weeks. Then the ratio were significantly decreased after transplantation MMSCs 8 weeks. Thirdly, our results also demonstrated that the density of microvasculars were increased at the boundary of infarction site in the animals transplanted MMSCs. Finally, total volume of the myocardial fibrillar collagen was reduced in the MMSCs treated groups after MI. Conclusion Transplanting MMSCs can improve the ventricular remodeling and heart functions to acute MI rat models.

Key words: Myocardial infarction, Marrow mesenchymal stem cells, Ventricular remodeling, Enhanced green fluorescent protein

伍徐娴何志旭沈祥春周中军刘鉴. 移植MMSCs改善大鼠实验性心肌梗死后心室重构[J]. 基础医学与临床, 2010, 30(1): 48-53.

Xu-xian WU; Zhi-xu HE; Xiang-chun SHEN; Zhong-jun ZHOU; Jian LIU. Transplanting Marrow Mesenchymal Stem Cells can Improve the Ventricular Remodeling and Heart Functions to Acute MI Rat Models[J]. Basic & Clinical Medicine, 2010, 30(1): 48-53.

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