沙利度胺抑制TGF-β1诱导的人胚肺成纤维细胞系转分化为肌成纤维细胞

基础医学与临床 ›› 2009, Vol. 29 ›› Issue (1): 59-63.

沙利度胺抑制TGF-β1诱导的人胚肺成纤维细胞系转分化为肌成纤维细胞

王峙赵丽丹张烜唐福林于孟学

中国医学科学院中国协和医科大学北京协和医院风湿免疫科中国医学科学院中国协和医科大学北京协和医院风湿免疫科中国医学科学院中国协和医科大学北京协和医院风湿免疫科中国医学科学院中国协和医科大学北京协和医院风湿免疫科中国医学科学院中国协和医科大学北京协和医院风湿免疫科

收稿日期:2007-10-30 修回日期:2008-04-28 出版日期:2009-01-25 发布日期:2009-01-25
通讯作者: 张烜

Thalidomide inhibits TGF-β1－induced transdifferentiation of human fetal lung fibroblast cell line to myofibroblast

Zhi WANG, Li-dan ZHAO, Xuan ZHANG, Fu-lin TANG, Meng-xue YU

Department of Rheumatology，PUMC Hospital，CAMS&PUMC Department of Rheumatology，PUMC Hospital，CAMS&PUMC Department of Rheumatology，PUMC Hospital，CAMS&PUMC Department of Rheumatology，PUMC Hospital，CAMS&PUMC Department of Rheumatology，PUMC Hospital，CAMS&PUMC

Received:2007-10-30 Revised:2008-04-28 Online:2009-01-25 Published:2009-01-25
Contact: Xuan ZHANG,

摘要/Abstract

摘要： 目的研究沙利度胺(THD)对转化生长因子β1(TGF-β1)诱导的人胚肺成纤维细胞(HFL-F)向肌成纤维细胞(MF)转分化和对已分化MF的作用。方法体外培养HFL-F，以TGF-β1(5μg/L)诱导HFL-F向MF转分化，通过碱水解法测定羟脯氨酸含量(Hyp)，免疫印迹法测定α-平滑肌肌动蛋白(α-SMA)表达，半定量RT-PCR测定α-SMA mRNA和Ⅲ型胶原(COL Ⅲ)mRNA,观察THD(50μg/L)对HFL-F向MF转分化的过程及已分化MF的影响。结果 TGF-β1诱导HFL-F 96h，诱导分化同时加入THD可抑制Hyp含量增高和COL Ⅲ mRNA表达上调(p均<0.05)，可抑制α-SMA蛋白表达增加，但对于α-SMA mRNA表达没有影响。TGF-β1诱导HFL-F 96h后再加入THD可抑制COL Ⅲ mRNA的表达(p<0.05),但对Hyp含量、α-SMA蛋白表达量和α-SMA mRNA表达无明显影响。结论 TGF-β1可诱导HFL-F向MF转分化，THD可抑制这一过程中α-SMA蛋白与胶原合成。对TGF-β1诱导下已分化的MF，THD可抑制其COL Ⅲ mRNA的表达。

Abstract: Objective To investigate the effects of Thalidomide（THD）on transdifferentiation of human fetal lung fibroblast (HFL-F) to myofibroblast (MF) induced by Transforming Growth Factor-β1 (TGF-β1) and on differentiated MF. Methods HFL-F to MF differentiation was induced with 5μg/L TGF-β1. The effect of 50μg/L THD on HFL-F to MF transdifferentiation was evaluated by measuring hydroxyproline (Hyp) content with alkaline hydrolysis method, α-smooth muscle actin (α-SMA) protein with Western Blot, α-SMA and collagen Ⅲ (COL Ⅲ) mRNA with semiquantitative RT-PCR. Results THD could inhibit TGF-β1 induced up-regulation of Hyp and COL Ⅲ mRNA expressions (all p<0.05), α-SMA protein synthesis may reduced, but had no effect on α-SMA mRNA expression (p>0.05). For HFL-F treated with 5μg/L TGF-β1 for 96h, THD could inhibit COL Ⅲ mRNA expression (p<0.05). Conclusions TGF-β1 could induce HFL-F to MF transdifferentiation; THD could affect this process by inhibiting Hyp production, α-SMA protein synthesis and COL Ⅲ mRNA expression. THD could additionally inhibit COL Ⅲ mRNA expression in transdifferentiated MF.

王峙赵丽丹张烜唐福林于孟学. 沙利度胺抑制TGF-β1诱导的人胚肺成纤维细胞系转分化为肌成纤维细胞[J]. 基础医学与临床, 2009, 29(1): 59-63.

Zhi WANG; Li-dan ZHAO; Xuan ZHANG; Fu-lin TANG; Meng-xue YU. Thalidomide inhibits TGF-β1－induced transdifferentiation of human fetal lung fibroblast cell line to myofibroblast[J]. Basic & Clinical Medicine, 2009, 29(1): 59-63.