Abstract: A variety of heat shock protein 70(HSP70)-associated therapeutic vaccines have been developed in anti-tumor immunotherapy, including protein vaccine, peptide vaccine, tumor cell vaccine and DNA vaccine, etc, in which HSP70-fused DNA vaccine attracts our attention with its superiority, it not only induces consistent anti-tumor immune response but also helps sustain immune memory. In this article we reviewed the function and application of HSP70 in the therapeutic tumor DNA vaccine.