复方倍他米松增加流感病毒感染小鼠受损嗅粘膜嗅觉标记蛋白表达

基础医学与临床 ›› 2010, Vol. 30 ›› Issue (5): 530-533.

复方倍他米松增加流感病毒感染小鼠受损嗅粘膜嗅觉标记蛋白表达

万桂莲倪道凤关静

中国医学科学院北京协和医学院北京协和医院耳鼻咽喉科中国医学科学院北京协和医学院北京协和医院耳鼻咽喉科

收稿日期:2010-01-27 修回日期:2010-03-12 出版日期:2010-05-05 发布日期:2010-05-05
通讯作者: 倪道凤

Compound Betamethasone enhances the expression of olfactory marker protein in the injured olfactory mucosa of mice by influenza virus

Gui-lian WAN, Dao-feng NI, Jing GUAN

Department of Otorhinolaryngology,PUMC Hospital,CAMS＆PUMC Department of Otorhinolaryngology,PUMC Hospital,CAMS＆PUMC

Received:2010-01-27 Revised:2010-03-12 Online:2010-05-05 Published:2010-05-05
Contact: Dao-feng NI,

摘要/Abstract

摘要： 目的探讨复方倍他米松对流感病毒感染小鼠受损的嗅粘膜嗅觉标记蛋白（OMP）表达的影响。方法模拟建立流感病毒性嗅觉障碍小鼠模型，分别于第2天和第4天给予复方倍他米松干预，腹腔内注射复方倍他米松，采用免疫组化和western blot 的方法检测嗅粘膜OMP蛋白的表达水平。结果流感病毒感染1组和2组嗅粘膜OMP的表达水平下调，流感病毒感染后复方倍他米松干预1组和2组嗅粘膜OMP的表达水平明显上调。结论复方倍他米松能够增加流感病毒损伤的嗅粘膜OMP的表达。

关键词: 糖皮质激素, 流感病毒, 嗅粘膜, 嗅觉标记蛋白

Abstract: Objective To investigate the effects of Compound Betamethasone on the expression of olfactory marker protein(OMP) in murine injured olfactory mucosa by influenza virus. Method An animal model was produced by intranasal application of influenza virus to mice. Compound Betamethasone was respectively injected i.p.( 3.5mg/kg) on day 2 and day 4 after the insult. The expression of OMP was tested by immunohistochemistry and western blot. Results The expression level of OMP was significantly downregulated in the olfactory mucosa of influenza virus control group 1 and influenza virus control group 2; the expression level of OMP was significantly upregulated in the olfactory mucosa of post-virus Compound Betamethasone group 1 and post-virus Compound Betamethasone group 2. Conclusion Compound Betamethasone can enhance The expression of OMP in the injured olfactory mucosa by influenza virus.

Key words: glucocorticoid, influenza virus, olfactory mucosa, olfactory marker protein

中图分类号:

R765.6

万桂莲倪道凤关静. 复方倍他米松增加流感病毒感染小鼠受损嗅粘膜嗅觉标记蛋白表达[J]. 基础医学与临床, 2010, 30(5): 530-533.

Gui-lian WAN; Dao-feng NI; Jing GUAN. Compound Betamethasone enhances the expression of olfactory marker protein in the injured olfactory mucosa of mice by influenza virus[J]. Basic & Clinical Medicine, 2010, 30(5): 530-533.

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