基础医学与临床 ›› 2010, Vol. 30 ›› Issue (5): 476-479.

• 研究论文 • 上一篇    下一篇

低氧无血清诱导乳鼠心肌细胞胰岛素样生长因子2受体表达上调

张秀芳 史强 崔传珏 李君 魏英杰 胡盛寿   

  1. 北京协和医学院 中国医学科学院 阜外心血管病医院卫生部心血管病疾病再生医学重点实验室 中国医学科学院 心血管病研究所 北京协和医学院 阜外心血管病医院 卫生部心血管疾病再生医学重点实验室 中国医学科学院北京协和医学院阜外心血管病医院 北京协和医学院 中国医学科学院 阜外心血管病医院卫生部心血管疾病再生医学重点实验室 中国医学科学院 北京协和医学院 阜外心血管病医院
  • 收稿日期:2009-10-09 修回日期:2009-11-16 出版日期:2010-05-05 发布日期:2010-05-05
  • 通讯作者: 魏英杰

Upregulated expression of IGF-2R induced by hypoxia and serum deprivation in neonatal rat cardiomyocytes in vitro

Xiu-fang ZHANG, Qiang SHI, Chuan-jue CUI, Jun LI, Ying-jie WEI, Sheng-shou HU   

  1. Key Laboratory of Cardiovascular Regenerative Medicine,Cardiovascular Institute and Fu Wai Hospital, CAMS & PUMC Key Laboratory of Cardiovascular Regenerative Medicine,Cardiovascular Institute and Fu Wai Hospital, CAMS & PUMC Cardiovascular Institute and Fu-Wai Hospital, PUMC & CAMS
  • Received:2009-10-09 Revised:2009-11-16 Online:2010-05-05 Published:2010-05-05
  • Contact: Ying-jie WEI,

摘要: 目的 研究胰岛素样生长因子2受体(IGF-2R)在体外模拟心肌缺血低氧环境时表达的变化。方法 分离培养SD乳鼠心肌细胞,以低氧和无血清处理不同时间点后收获细胞,用反转录聚合酶链式反应(RT-PCR)和实时定量RT-PCR测定IGF-2R mRNA的表达,用酶联免疫吸附法(ELISA)测定IGF-2R蛋白水平。结果 与对照组相比,低氧无血清处理12h和24h乳鼠心肌细胞IGF-2R mRNA表达显著增高(P<0.01),处理24h后IGF-2R蛋白表达也升高(P<0.05)。结论 低氧无血清可诱导乳鼠心肌细胞IGF-2R的表达,IGF-2R可能在缺血低氧引起心肌损害过程中发挥重要的作用。

关键词: 胰岛素样生长因子2受体, 缺血缺氧, 心肌细胞

Abstract: Objective To investigate the expression of insulin-like growth factor 2 receptor (IGF-2R) induced by hypoxia and serum deprivation (H/SD) in cultured neonatal rat cardiomyocytes in vitro. Methods Cultured neonatal rat cardiomyocytes were randomly divided into control group and H/SD group. Expression of IGF-2R mRNA was measured by RT-PCR and real time RT-PCR. Level of IGF-2R protein was measured by enzyme-linked immunosorbent assay (ELISA). Results Compared with control group, the expression of IGF-2R mRNA increased significantly (P<0.01) in cardiomyocytes cultured in H/SD condition for 12h and 24h, and IGF-2R protein was upregulated in cardiomyocytes cultured in H/SD condition for 24h(P<0.05). Conclusion H/SD could induce IGF-2R expression, and IGF-2R may involve in the mechanism of cardiomyocyte damage induced by ischemia.

Key words: IGF-2R, Hypoxia and Serum deprivation, cardiomyocytes