关键词: 阿尔茨海默病, Aβ多肽, T细胞亚型, 细胞因子

Abstract: Objective To observe stimulation and regulation of T lymphocyte subtypes when using wild-type and mutant Aβ40 and Aβ42 with different adjuvants and to investigate possible ways to lower the toxicity of Aβ immune response. Methods Forty BALB/c mice were randomized into five groups: Al adjuvant group,Aβ42+CFA group,Aβ42+Al group,Aβ40+Al group,Aβ40（E22A）+Al group. After the initial immunization and booster immunization 3 times, antibody titer were detected and the mice were killed. Their splenocytes were stimulated by respective antigen. After 48h quantity of IFN-γ, IL-2, TNF-α and IL-4 were tested. After 72h of culture, the proliferative response of splenocytes were detected by CCK-8 assay. Results Experimental groups generated specific anti-Aβ antibodies and had proliferative response of spleen lymphocytes. Cell culture supernatant of cytokine detection results showed that Aβ42+CFA group had the highest secretion of Th1-type cytokines IFN-γ,IL-2,TNF-α when Aβ40（E22A）+Al group had the lowest.(P <0.01).When Aβ40 and the mutant Aβ40+Al groups compared with Aβ42+Al group, secretion of IFN-γ,IL-2,TNF-α have also decreased significantly（P＜0.05）.The secretion of IL-4 was no significant difference. Conclusion Aβ40（E22A）+Al group stimulate the lowest toxicity of T cells, and may have better security in the immune response.

Key words: Alzheimer’s disease, Aβ peptide, T cell subtypes, cytokine