Abstract: Objective To study the expression changes and metabolic effects of DBP, RBP and TTR after acute pulmonary embolism(PE) in a rat model. Methods We constructed a rat acute PE model by injecting emboli into the jugular vein. The serum protein levels of DBP, RBP and TTR were validated by western blot methods. The mRNA levels of DBP, RBP and TTR were identified by semi-quantitative RT-PCR. The serum concentrations of 25(OH)D3 and FT4 were determined by means of RIA, and that of Vitamin A were checked by microfluorescence. Results The serum protein levels of DBP, RBP and TTR and their mRNA levels in rat liver all decreased gradually after acute PE. On the contrary, the serum concentrations of 25(OH)D3, Vitamin A and FT4 increased sharply after acute PE. Conclusion The synthesis of DBP, RBP and TTR in rat liver was depressed obviously after acute PE, which resulted in the down-regulation of the serum protein levels of them. The low serum concentrations of DBP, RBP and TTR would lead to the release of their ligands------25(OH)D3, Vitamin A and FT4, which would play important roles in the pathophysiologic changes after acute PE.