麦角硫因对中老年小鼠组织代谢的影响

doi:10.16352/j.issn.1001-6325.2025.06.0709

基础医学与临床 ›› 2025, Vol. 45 ›› Issue (6): 709-713.doi: 10.16352/j.issn.1001-6325.2025.06.0709

麦角硫因对中老年小鼠组织代谢的影响

陈浩然, 王芳, 马艳妮, 余佳, 王琳^*

中国医学科学院基础医学研究所北京协和医学院基础学院生物化学与分子生物学系重大疾病共性机制研究全国重点实验室,北京 100005

收稿日期:2025-02-21 修回日期:2025-03-21 出版日期:2025-06-05 发布日期:2025-05-26
通讯作者: ^*linwang@ibms.pumc.edu.cn
基金资助:
国家重点研发计划(2022YFA1106300)

Effect of ergothioneine on tissue metabolism in middle-aged and aged mice

CHEN Haoran, WANG Fang, MA Yanni, YU Jia, WANG Lin^*

State Key Laboratory of Common Mechanism Research for Major Diseases, Department of Biochemistry and Molecular Biology, Institute of Basic Medicine CAMS, School of Basic Medicine PUMC, Beijing 100005, China

Received:2025-02-21 Revised:2025-03-21 Online:2025-06-05 Published:2025-05-26

摘要/Abstract

摘要： 目的探究麦角硫因(EGT)对中老年小鼠组织代谢的影响。方法选取9个月龄的中老年C57BL/6J小鼠,实验组按照35 mg EGT/kg体质量的剂量进行EGT水溶液灌胃,对照组进行等量的水灌胃,每两天干预1次,两组小鼠均用不含EGT的饲料喂养。持续灌胃7周后收集小鼠的肝脏、肾、小肠组织,借助超高效液相色谱-质谱(UHPLC-MS)技术进行非靶向代谢组学检测。挖掘差异代谢物并进行KEGG代谢通路富集分析。结果与对照组相比,经EGT灌胃处理后的中老年小鼠的肝脏、肾、小肠组织中氧化还原平衡相关的代谢物丰度未发生明显改变,但肝脏中(次)牛磺酸代谢(P＜0.01)、肾中嘌呤代谢(P＜0.01)、小肠中半胱氨酸和甲硫氨酸代谢(P＜0.001)等受到了显著影响。结论麦角硫因作用下中老年小鼠肝脏、肾、小肠组织中发生了非氧化还原相关的代谢变化。

关键词: 麦角硫因, 中老年小鼠, 代谢组, 代谢通路

Abstract: Objective To investigate the effect of ergothioneine(EGT) on tissue metabolism in middle-aged and aged mice. Methods Nine-month-old middle-aged and aged C57BL/6J mice were selected to be gavaged with EGT aqueous solution at the dose and frequency of 35 mg EGT per kg body weight every two days. The control group was gavaged with the same amount of water. Both groups were fed with EGT-free diet. After 7 weeks, the liver, kidney, and small intestine of mice were collected, and untargeted metabolomics analysis was performed using ultra-performance liquid chromatography-mass spectrometry(UHPLC-MS). The differential metabolites were selected for KEGG metabolic pathway enrichment analysis. Results Compared with the control group, the abundances of metabolites related to redox balance in liver, kidney and small intestine of middle-aged and aged mice treated with EGT did not change significantly. However, taurine and hypotaurine metabolism in liver(P＜0.01), purine metabolism in kidney(P＜0.01), and cysteine and methionine metabolism in small intestine(P＜0.001) were affected. Conclusions Non-redox-related metabolic changes in the liver, kidney and small intestine of middle-aged and aged mice by ergothioheine.

Key words: ergothioneine, middle-aged and aged mice, metabolome, metabolic pathways

中图分类号:

Q591.9

陈浩然, 王芳, 马艳妮, 余佳, 王琳. 麦角硫因对中老年小鼠组织代谢的影响[J]. 基础医学与临床, 2025, 45(6): 709-713.

CHEN Haoran, WANG Fang, MA Yanni, YU Jia, WANG Lin. Effect of ergothioneine on tissue metabolism in middle-aged and aged mice[J]. Basic & Clinical Medicine, 2025, 45(6): 709-713.

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麦角硫因对中老年小鼠组织代谢的影响

Effect of ergothioneine on tissue metabolism in middle-aged and aged mice

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