核酸适配体W3介导靶向乳腺癌的量子点成像

doi:10.16352/j.issn.1001-6325.2023.01.0076

基础医学与临床 ›› 2023, Vol. 43 ›› Issue (1): 76-82.doi: 10.16352/j.issn.1001-6325.2023.01.0076

核酸适配体W3介导靶向乳腺癌的量子点成像

王群, 李鑫, 马俊, 李婉明^*

中国医科大学卫生部细胞生物学重点实验室医学细胞生物学教育部重点实验室分子细胞生物学教研室,辽宁沈阳 110122

收稿日期:2022-02-23 修回日期:2022-06-02 出版日期:2023-01-05 发布日期:2022-12-27
通讯作者: ^*wmli@cmu.edu.cn
基金资助:
辽宁省自然科学基金(2020-MS-164);沈阳市中青年科技创新人才支持计划(RC190261)

Imaging of breast cancer targeted by the nucleic acid aptamer W3-quantum dots

WANG Qun, LI Xin, MA Jun, LI Wanming^*

Department of Molecular Cell Biology, Key Laboratory of Medical Cell Biology, Ministry of Education, Key Laboratory of Cell Biology, Ministry of Public Health, China Medical University, Shenyang 110122, China

Received:2022-02-23 Revised:2022-06-02 Online:2023-01-05 Published:2022-12-27
Contact: ^*wmli@cmu.edu.cn

摘要/Abstract

摘要： 目的利用核酸适配体W3对乳腺癌细胞的结合特异性,对乳腺癌细胞以及临床乳腺癌组织石蜡切片标本进行量子点(QDs)成像。方法利用流式细胞测量术检测核酸适配体W3对乳腺癌细胞系的结合特异性,基于生物素与链霉亲和素的结合反应原理,通过荧光显微镜观察核酸适配体W3对乳腺癌细胞系以及临床乳腺癌患者组织标本的量子点靶向成像,并进一步分析其临床意义。结果核酸适配体W3能特异性结合恶性程度较高的乳腺癌细胞,偶联量子点QD605形成量子点探针(W3-QDs),能够对恶性程度较高的乳腺癌细胞系以及乳腺癌组织实现靶向成像,并且具有高恶性程度的乳腺癌组织的荧光强度明显高于恶性程度低的乳腺癌组织。结论核酸适配体W3偶联量子点后能够实现乳腺癌的靶向成像,可用于乳腺癌的早期诊断。

关键词: 适配体, 乳腺癌, 量子点, 靶向成像

Abstract: Objective To perform quantum dots(QDs)imaging of breast cancer cells and breast cancer tissue paraffin specimens by using nucleic acid aptamer W3 binding specificity on breast cancer cells. Methods The binding specificity of nucleic acid aptamer W3 to breast cancer cell lines was detected by flow cytometry, and based on the binding reaction principle of biotin and streptomyces avidin, the quantum dot targeting imaging of nucleic acid aptamer W3 on breast cancer cell lines and tissue specimens of clinical patients was observed by fluorescence microscopy, and its clinical significance was further analyzed. Results The nucleic acid aptamer W3 specifically binded to breast cancer cell lines with high malignancy,and after coupling quantum dots QD605(W3-QDs), it achieved targeted imaging of breast cancer cell lines and breast cancer tissues with high malignancy, and the fluorescence intensity of breast cancer tissues with high malignancy degree was significantly higher than that of breast cancer tissues with low malignancy. Conclusions The nucleic acid aptamer W3 may achieve target imaging of breast cancer after coupling quantum dots, which can be used for early diagnosis of breast cancer.

Key words: aptamer, breast cancer, quantum dot, targeted imaging

中图分类号:

王群, 李鑫, 马俊, 李婉明. 核酸适配体W3介导靶向乳腺癌的量子点成像[J]. 基础医学与临床, 2023, 43(1): 76-82.

WANG Qun, LI Xin, MA Jun, LI Wanming. Imaging of breast cancer targeted by the nucleic acid aptamer W3-quantum dots[J]. Basic & Clinical Medicine, 2023, 43(1): 76-82.

参考文献 14

[1]	Harbeck N, Gnant M. Breast cancer[J]. Lancet, 2017, 389:1134-1150.
[2]	Conti A, Duggento A, Indovina I, et al. Radiomics in breast cancer classification and prediction[J]. Semin Cancer Biol, 2021, 72:238-250.
[3]	李晓欧, 胡燕, 安雅聪, 等.针对于白蛋白的新型核酸适配体的筛选与鉴定[J]. 基础医学与临床, 2017, 37:935-938.
[4]	Wang S, Li W, Yuan D, et al. Quantitative detection of the tumor-associated antigen large external antigen in colorectal cancer tissues and cells using quantum dot probe[J]. Int J Nanomedicine, 2016, 11:235-247.
[5]	Liang Z, Khawar MB, Liang J, et al. Bio-Conjugated quantum dots for cancer research: detection and imaging[J]. Front Oncol, 2021, 11:749970. doi:10.3389/fonc.2021.749970.
[6]	Matea CT, Mocan T, Tabaran F, et al. Quantum dots in imaging, drug delivery and sensor applications[J]. Int J Nanomedicine, 2017, 12:5421-5431.
[7]	Li WM, Bing T, Wei JY, et al. Cell-SELEX-based selection of aptamers that recognize distinct targets on metastatic colorectal cancer cells[J]. Biomaterials, 2014, 35:6998-7007.
[8]	Bauer M, Strom M, Hammond DS, et al. Anything you can do, I can do better: Can aptamers replace antibodies in clinical diagnostic applications?[J]. Molecules, 2019, 24:4377. doi:10.3390/molecules24234377.
[9]	Ni S, Zhuo Z, Pan Y, et al. Recent progress in aptamer discoveries and modifications for therapeutic applications[J]. ACS Appl Mater Interfaces, 2021, 13:9500-9519.
[10]	Zeng Z, Zhang P, Zhao N, et al. Using oligonucleotide aptamer probes for immunostaining of formalin-fixed and paraffin-embedded tissues[J]. Mod Pathol, 2010, 23: 1553-1558.
[11]	Kundu S, Ghosh M, Sarkar N. State of the art and perspectives on the biofunctionalization of fluorescent metal nanoclusters and carbon quantum dots for targeted imaging and drug delivery[J]. Langmuir, 2021, 37:9281-9301.
[12]	Leonel AG, Mansur AAP, Mansur HS. Advanced functional nanostructures based on magnetic iron oxide nanomaterials for water remediation: a review[J]. Water Res, 2021, 190:116693. doi:10.1016/j.watres.2020.116693.
[13]	Díaz-González M, Escosura-Muñiz A, Fernandez-Argüelles MT, et al. Quantum dot bioconjugates for diagnostic applications[J]. Top Curr Chem, 2020, 378:35. doi:10.1007/s41061-020-0296-6.
[14]	Zhao T, Fu Y, Jang MS, et al. A pH-activated charge convertible quantum dot as a novel nanocarrier for targeted protein delivery and real-time cancer cell imaging [J]. Mater Sci Eng C Mater Biol Appl, 2021, 118:111449. doi:10.1016/j.msec.2020.111449.

[1]	苏凌峰, 王虎霞, 王延峰, 宋张骏. 慢性压力对乳腺癌发展的影响[J]. 基础医学与临床, 2024, 44(9): 1303-1307.
[2]	夏雨佳, 杨振丽, 代娣, 刘玉琴. 乳腺癌类器官的培养及鉴定[J]. 基础医学与临床, 2024, 44(9): 1223-1228.
[3]	陆凯, 陆建菊, 郭文利, 黄建棋, 李志华. lncRNA VIM-AS5在人乳腺癌细胞系中的表达及对乳腺癌细胞增殖和迁移的影响[J]. 基础医学与临床, 2024, 44(4): 447-453.
[4]	南璐, 郭梦杰, 高雅楠, 贾红燕. 膜联蛋白A家族成员在乳腺癌中作用机制的研究进展[J]. 基础医学与临床, 2024, 44(3): 393-397.
[5]	依合里曼·买买提, 曹燕珍, 王翠翠, 岳娜, 梁莉萍. PIK3CA突变与乳腺癌临床病理特征及预后的相关性[J]. 基础医学与临床, 2024, 44(3): 303-307.
[6]	李江丽, 孙静, 唐一君, 郭君兰, 陈博, 郭胜男. Circ-DDX5靶向miR-3940抑制人乳腺癌细胞系的增殖和侵袭[J]. 基础医学与临床, 2024, 44(1): 1-7.
[7]	陈宁, 彭甜影, 张伟苗, 高亚诺, 李轩, 闫彩珍, 李军霞. 雷公藤甲素与阿霉素联用对人乳腺癌细胞系 MCF-7 的协同促凋亡作用[J]. 基础医学与临床, 2023, 43(8): 1186-1192.
[8]	王继辉, 曹吉烈, 张立军, 张军, 鹿文葆. TARBP2降解AKAP12转录本促进人乳腺癌细胞转移[J]. 基础医学与临床, 2023, 43(8): 1259-1264.
[9]	唐一君, 王文龙, 李江丽, 杨欢, 孙静. LncRNA RP11-686D22.10与乳腺癌患者生存期相关并影响乳腺癌细胞系的增殖和侵袭[J]. 基础医学与临床, 2023, 43(7): 1023-1029.
[10]	杨良权, 于淼, 姜茜, 张明慧, 杨海松. miR-504调控蛋白酶体激活因子抑制人乳腺癌细胞系MCF-7增殖及迁移[J]. 基础医学与临床, 2023, 43(1): 95-101.
[11]	张晓雷, 刘静, 李朝萍. Circ_0032821参与索拉非尼抑制乳腺癌细胞系T47D增殖、迁移和侵袭[J]. 基础医学与临床, 2022, 42(9): 1350-1354.
[12]	王子辕, 段昭君, 罗云萍. 小鼠乳腺癌肺特异性转移细胞亚系的建立和验证[J]. 基础医学与临床, 2022, 42(7): 1083-1091.
[13]	韩雪莹, 崔丰, 李隽. 乳腺癌缺失因子1(DBC1)影响乳腺癌细胞系对依托泊苷的敏感性[J]. 基础医学与临床, 2022, 42(7): 1060-1066.
[14]	康有力, 赵丹, 杜文静, 李莉. SIRT5通过调控ALDH5A1的琥珀酰化促进人乳腺癌细胞的增殖[J]. 基础医学与临床, 2022, 42(7): 1099-1107.
[15]	谢利华, 杨奇, 李雅倩, 谢心悦, 雷诗娟, 周琳. 转录因子Kaiso下调人乳腺癌细胞系中lncRNA MEG3的表达[J]. 基础医学与临床, 2022, 42(5): 758-762.

核酸适配体W3介导靶向乳腺癌的量子点成像

Imaging of breast cancer targeted by the nucleic acid aptamer W3-quantum dots

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献 14

相关文章 15

编辑推荐

Metrics

本文评价