关键词: miR-493-5p, GIT1, MEK1/2-ERK1/2通路, 卵巢癌, 侵袭

Abstract: Objective To investigate the effects of miR-493-5p on proliferation, invasion and migration of ovarian cancer cells and underlying mechanism. Methods Culture human normal ovarian epithelial cell lines IOSE80 and ovarian cancer cell lines A2780, SKOV3, OVCAR3, transfected the miR-493-5p over-expression vectors, miR-493-5p suppressed expression vectors and G protein coupled receptor kinase interacting factor 1 (GIT1) over-expression vectors into SKOV3 cells; RT-qPCR was used to detect miR-493-5p and GIT1 mRNA expression; Western blot was used to detect protein expression; MTT assay was used to detect cell viability; Transwell was used to detect cell migration and invasion; dual luciferase report experiment was applied for detection of the targeting relationship between miR-493-5p and GIT1. Results In ovarian cancer cells A2780, SKOV3, OVCAR3, miR-493-5p were weakly expressed, and GIT1 was highly expressed (P＜0.05); After over-expression of miR-493-5p, the proliferation activity, migration and invasion number of ovarian cancer cell SKOV3 were decreased (P＜0.05). miR-493-5p targeted at the regulation of GIT1 expression, and over-expression of GIT1 reduced the inhibitory effect of miR- 493-5p on proliferation, migration and invasion of ovarian cancer cell SKOV3 and expression of p-MEK1/2, p-ERK1/2, pc-Jun and pc-Fos. Conclusions miR-493-5p can inhibit the proliferation, migration and invasion of ovarian cancer cells SKOV3. The mechanism may be related to the targeted regulation of GIT1 and MEK1/2-ERK1/2 pathway, which thus provides some new targets for diagnosis, prevention and treatment for ovarian cancer.

Key words: miR-493-5p, GIT1, MEK1/2-ERK1/2 pathway, ovarian cancer, invasion