miR-26a靶向IGF-1抑制子宫肌瘤细胞增殖、迁移和侵袭

基础医学与临床 ›› 2021, Vol. 41 ›› Issue (11): 1612-1617.

miR-26a靶向IGF-1抑制子宫肌瘤细胞增殖、迁移和侵袭

向雪芹¹, 崔权哲², 童玉娜³, 詹文斌¹, 陆静^1*

成都市第三人民医院 1.妇产科; 2.病理科; 3.药剂科, 四川成都 610014

收稿日期:2021-02-03 修回日期:2021-08-06 发布日期:2021-10-27
通讯作者: *1078301319@qq.com
基金资助:
四川省卫生和计划委员会科研项目(16PJ046)

miR-26a inhibits the proliferation, migration and invasion of uterine leiomyoma cells through targeting IGF-1

XIANG Xue-qin¹, CUI Quan-zhe², TONG Yu-na³, ZHAN Wen-bin¹, LU Jing^1*

1. Department of Obstetrics and Gynecology; 2. Department of Pathology;3. Department of Pharmacy, the Third People's Hospital of Chengdu, Chengdu 610014, China

Received:2021-02-03 Revised:2021-08-06 Published:2021-10-27
Contact: *1078301319@qq.com

摘要/Abstract

摘要： 目的探讨miR-26a靶向胰岛素样生长因子1基因(IGF-1)对子宫肌瘤细胞增殖、迁移和侵袭的影响及作用机制。方法将原代培养的子宫肌瘤细胞分为miR-NC组(转染mimics阴性对照序列)、miR-26a组(转染miR-26a模拟物mimics)、(miR-26a+pcDNA3.1)组(共转染miR-26a mimics及空载体)和(miR-26a+IGF-1)组(共转染miR-26a mimics及IGF-1过表达载体)。RT-qPCR检测miR-26a表达;MTT法及Transwell小室法分别检测细胞增殖和迁移和侵袭;Western blot检测细胞增殖细胞核抗原(PCNA)、E-钙黏蛋白(E-cadherin)和基质金属蛋白酶2(MMP-2)表达;双荧光素酶报告基因实验验证miR-26a和IGF-1靶向关系。结果 miR-26a组子宫肌瘤细胞miR-26a表达明显升高(P＜0.05)。与miR-NC组比较,miR-26a组细胞增殖、迁移和侵袭能力明显降低,PCNA和MMP-2表达明显降低,而E-cadherin表达明显升高(P＜0.05)。miR-26a和IGF-1存在靶向关系。过表达IGF-1可减弱miR-26a对子宫肌瘤细胞增殖、迁移和侵袭能力的降低作用,减弱对PCNA和MMP-2表达的抑制作用(P＜0.05),减弱对E-cadherin表达促进作用(P＜0.05)。结论过表达miR-26a可通过靶向IGF-1抑制子宫肌瘤细胞增殖、迁移和侵袭。

关键词: 子宫肌瘤, miR-26a, 胰岛素样生长因子1, 迁移和侵袭

Abstract: Objective To investigate the effect and mechanism of miR-26a which targets at insulin like growth factor 1(IGF-1) on the proliferation, migration and invasion of uterine leiomyoma cells. Methods The primary cultured uterine leiomyoma cells were divided into miR-NC group(mimics negative control sequence and was transfected into cells), miR-26a group(miR-26a mimics was transfected into cells), (miR-26a+pcDNA3.1) group(miR-26a mimics and blank vector were transfected into cells) and (miR-26a + IGF-1) group(miR-26a mimics and IGF-1 over-expression vector were transfected into cells). The expression of miR-26a was detected by RT-qPCR; MTT method and Transwell chamber were used to detect cell proliferation, invasion and migration. Western blot was used to detect the expression of PCNA, E-cadherin and MMP-2. Dual luciferase reporter gene assay verified the targeting relationship between miR-26a and IGF-1. Results The miR-26a expression of uterine leiomyoma cells in miR-26a group was significantly increased (P＜0.05). Compared with miR-NC group, the cell proliferation, migration and invasion in miR-26a group decreased significantly, the expression of PCNA and MMP-2 decreased significantly, while the expression of E-cadherin increased significantly (P＜0.05). There was a targeting relationship between miR-26a and IGF-1. Over-expression of IGF-1 attenuated the inhibition of miR-26a on the proliferation, migration and invasion of uterine leiomyoma cells and the expression of PCNA and MMP-2, and promoting effect of E-cadherin expression. Conclusions Over-expression of miR-26a may inhibit proliferation, migration and invasion of uterine leiomyoma cells by targeting at IGF-1.

Key words: uterine leiomyoma, miR-26a, insulin like growth factor 1, migration and invasion

中图分类号:

R737.33

向雪芹, 崔权哲, 童玉娜, 詹文斌, 陆静. miR-26a靶向IGF-1抑制子宫肌瘤细胞增殖、迁移和侵袭[J]. 基础医学与临床, 2021, 41(11): 1612-1617.

XIANG Xue-qin, CUI Quan-zhe, TONG Yu-na, ZHAN Wen-bin, LU Jing. miR-26a inhibits the proliferation, migration and invasion of uterine leiomyoma cells through targeting IGF-1[J]. Basic & Clinical Medicine, 2021, 41(11): 1612-1617.

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