在不同转移潜能人肺癌细胞移植瘤中血管性血友病因子的表达

基础医学与临床 ›› 2020, Vol. 40 ›› Issue (3): 346-350.

在不同转移潜能人肺癌细胞移植瘤中血管性血友病因子的表达

司晓丽^1,2, 李双燕², 郭超¹, 张松³, 张立^1*

1.甘肃中医药大学基础医学院,甘肃兰州 730000;
2.兰州大学基础医学院,甘肃兰州 730000;
3.西北民族大学生物医学生物研究中心,甘肃兰州 730000

收稿日期:2019-03-18 修回日期:2019-10-09 出版日期:2020-03-05 发布日期:2020-03-02
通讯作者: ^*504530805@qq.com
基金资助:
甘肃省高等学校科研项目(2016A-042)甘肃省中医药管理局科研课题(GZK-2015-53)

Expression of vWF in transplanted human lung cancer with the different metastatic potential

SI Xiao-li^1,2, LI Shuang-yan², GUO Chao¹, ZHANG Song³, ZHANG Li^1*

1. Basic Medical College of Gansu University of Chinese Medicine, Lanzhou 730000;
2. Basic Medical College of Lanzhou University, Lanzhou 730000;
3. Biomedical Research Center of Northwest Minzu University, Lanzhou 730000, China

Received:2019-03-18 Revised:2019-10-09 Online:2020-03-05 Published:2020-03-02
Contact: ^*504530805@qq.com

摘要/Abstract

摘要： 目的探讨血管性血友病因子(vWF)在不同转移潜能人肺癌裸鼠移植瘤中的表达。方法体外培养低转移及高转移人肺癌细胞系A549和D95,Transwell小室法检测肿瘤细胞体外侵袭和迁移能力;10只Balb/c裸小鼠随机分为A549组和D95组(n=5)。0.2 mL两种肿瘤细胞悬液(浓度约为1×10⁷/mL)分别皮下注入A549组及D95组裸小鼠左上肢,构建人肺癌裸鼠移植瘤模型。观察vWF对裸小鼠移植瘤生长的影响;ELISA检测外周血vWF水平;免疫组化检测移植瘤vWF蛋白表达;免疫印记检测移植瘤、肺和肝vWF蛋白表达。结果 D95肺癌细胞体外侵袭和迁移细胞数多于A549肺癌细胞(P＜0.05);D95组裸小鼠于皮下接种后第5天全部出现瘤结节,A549组于第7天全部出现;与A549组相比,D95组裸小鼠移植瘤质量及肺转移瘤结节数显著增多(P＜0.05),D95组移植瘤、肺及肝组织vWF蛋白的表达显著高于A549组(P＜0.01)。结论 vWF与人肺癌细胞的转移潜能密切相关。

关键词: 血管性血友病因子, 转移潜能, 移植瘤

Abstract: Objective To explore the role of von Willebrand factor (hereinafter referred to as vWF) in the transplantation of human lung cancer with the different metastatic potential. Methods Low and high metastatic human lung cancer cell line A549 and D95 were cultured in vitro.The invasion and migration of lung cancer cells were detected by Transwell assay.Ten Balb/c nude mice were randomly divided into A549 group and D95 group(n=5).Then 0.2 mL of two tumor cell suspensions(the concentration was about 1×10⁷/mL) were subcutaneously injected respectively into the left upper limb of nude mice in A549 group and D95 group,to establish the xenograft model of human lung cancer in nude mice. The effects of vWF on transplantation tumor were analyzed.The level of vWF in peripheral blood was evaluated by ELISA.The expression of vWF protein in transplanted tumor was measured by immunohistochemistry.The expression of vWF protein in transplanted tumor, lung and liver was examined by Western blot. Results The number of invasion and migration of D95 lung cancer cells in vitro was more than that of A549 group(P＜0.05).After subcutaneous inoculation, all nude mice in the D95 group presented cancer nodules on the 5th day, and all nude mice in the A549 group did on the 7th day.Compared with the A549 group,the transplanted tumor mass and the quantity of lung metastatic tumor nodules in D95 group significantly increased(P＜0.05).Meanwhile,the expression of vWF protein in the transplanted tumor, lung and liver tissue were remarkably higher than that in the A549 group(P＜0.01). Conclusions vWF is closely related to the metastatic potential of human lung cancer cells.

Key words: vWF, metastatic potential, transplanted tumor

中图分类号:

R73-35⁺2

司晓丽, 李双燕, 郭超, 张松, 张立. 在不同转移潜能人肺癌细胞移植瘤中血管性血友病因子的表达[J]. 基础医学与临床, 2020, 40(3): 346-350.

SI Xiao-li, LI Shuang-yan, GUO Chao, ZHANG Song, ZHANG Li. Expression of vWF in transplanted human lung cancer with the different metastatic potential[J]. Basic & Clinical Medicine, 2020, 40(3): 346-350.

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