基础医学与临床 ›› 2019, Vol. 39 ›› Issue (1): 76-80.

• 研究论文 • 上一篇    下一篇

过表达骨形成发生蛋白4降低人胶质母细胞瘤系U251的侵袭

王璨,喻军华   

  1. 鄂州市中心医院
  • 收稿日期:2018-07-18 修回日期:2018-11-05 出版日期:2019-01-05 发布日期:2018-12-28
  • 通讯作者: 喻军华 E-mail:hbezyjh@126.com

Over-expression of bone morphogenetic protein 4 regulates invasion of human glioblastoma cell line U251

wang can1,   

  • Received:2018-07-18 Revised:2018-11-05 Online:2019-01-05 Published:2018-12-28

摘要: 目的: 探讨骨形成发生蛋白4(BMP4)对人胶质母细胞瘤细胞(U251)侵袭的影响。方法:将U251细胞,通过转染高表达BMP4的质粒及敲低BMP4的siRNA, 分为myc组、myc-BMP4组、siCtrl组、siBMP4组;用划痕实验、荧光定量PCR与免疫印迹技术,检测高/低表达BMP4后,细胞侵袭E-cadherin及Snail1mRNA和蛋白表达。结果:高表达BMP4降低细胞侵袭(P<0.01),低表达BMP4增加细胞侵袭。高表达BMP4后,Snail1信号通路靶基因E-cadherin表达量明显升高(P<0.01);低表达BMP4后 ,E-cadherin表达量明显降低(P<0.01);高表达BMP4 时,Snail1表达量明显降低(P<0.01)。结论:BMP4 可以通过负性调节Snail1信号通路调节细胞侵袭。

关键词: 侵袭, BMP4, Snail1

Abstract: Objective To investigate the effect of BMP4 on glioblastoma cell invasion and its mechanism. Methods In human malignant glioma cells U251, myc, myc-BMP4, siCtrl, siBMP4 was transfected into cells, and changes on the invasive ability of cells with high and low BMP4 expression were detected by Scratch wound healing assay, In mechanism study, RT-PCR and western blot assay were used to detect the expression of E-cadherin and Snail1 in four groups. Results Wound healing results showed that high BMP4 expression in U251 cells decreased cell invasion(P<0.01), and low BMP4 expression increased cell invasion; RT-EPCR and Western blot assay results showed that high BMP4 expression significantly increase E-cadherin expression(P<0.01), low BMP4 expression significantly decrease E-cadherin expression(P<0.01), when BMP4 expression was high, Snail1 protein expression was decreased(P<0.01). Conclusion: BMP4 can regulate GBM cell invasion through negative regulation of Snail1 signaling pathway.

Key words: invasion, BMP4, snail1