关键词: 三维生物打印, 兔肺成纤维细胞, 水凝胶结构体, 片段

Abstract: Objective To construct three dimensional(3D) structure lamella containing rabbit’s 1ung fibroblasts/alginate/gelatin blend using 3D bioprinting technique. Methods Rabbit’s 1ung fibroblasts were extracted from lung tissue pieces of newborn rabbit using collagenase digestion combined with tissue pieces culture method. Alginate and gelatin were mixed to form hydrosol,and rabbit’s 1ung fibroblasts were added into the hydroso1．A 3D bioprinting system was used to construct 3D printed like-lung structures using rabbit’s 1ung fibroblasts-alginate-gelatin blend.The like-lung hydrogel structures were cultured, and the cells’ survival rate was detected by live/dead cells double fluorescence staining. Results The 3D structures with the grid hydrogel composed of multilayer staggered cylindrical microfilament were obtained. The size of the structure was about 9 mm×9 mm×1.2 mm, and pore size was about 600 μm×600 μm. The results of live/dead cells fluorescence staining showed that the 1ung fibroblasts’ survival rate was 84%±3% after printing．Conclusions 3D bioprinting of 1ung fibroblasts is feasible，which provides a new way to construct like-lung tissue engineering．

Key words: rabbit’s 1ung fibroblasts, 3D bioprinting, hydrogel structure, lamella