关键词: 关键词：骨髓间充质干细胞, PFT-α, BMP-2, 心肌细胞

Abstract: Objective To investigate the effect of PFT- and BMP-2 on the differentiation of bone marrow mesenchymal stem cells (BMMSCs) into heart like cells. Methods Cells were isolated from rat bone marrow and cultured, passaged and purified method, PTF-a, BMP-2 and both were used to induce the second generation BMMSCs, and the inducer was not used as the control group.The application of inverted to observe the changes of growth and morphology of cells cultured by flow cytometry microscope, identified by BMMSCs combined with surface markers were detected by immunohistochemical method cTnI, Cx43 and TPM expression and ultrastructural changes were observed in the differentiated cells by transmission electron microscope.Results After induction, BMMSCs was spindle shaped, the volume of cells became larger.CD29(+)86.5%,CD45(-)0.3%, CD90(+)84.7%.The expression of cTnI,Cx43 and TPM in the induced group was positive. The expression of Cx43 in the cytoplasm of the induced group was red, and the expression of cTnI immunofluorescence staining was green.oval nucleus is located in the central cell, Golgi apparatus, mitochondria, rough endoplasmic reticulum and ribosome subcellular structures located in cytoplasm,parallel arrangement of filaments.Conclusions Both PFT- and BMP-2 can induce BMMSCs to differentiate into cardiomyocytes, and the combination of these two methods is safer and more efficient.

Key words: Key words:? bone?marrow?mesenchymal?stem?cells, ?p-fiftythree?inhibitor, bone morphogenetic? protein 2, ?cardiomyocytes