关键词: Islet-1, 组蛋白乙酰化, DNA甲基化, 间充质干细胞, 心肌细胞

Abstract: Objective To research the interaction between early myocardial developmental gene GATA4 histone acetylation and its DNA methylation of promoter during Islet-1 inducing the mesenchymal stem cells of C3H10T1/2 cells to differentiate specifically into cardiomyocytes.Method Constructing the model of Islet-1 over-expression cells,in the process of GATA4 gene expression by using chromatin immunoprecipitation(CHIP)to detecte histone H3K9 acetylation sequential changes of its promoter region and using the methylation-specific PCR(MSP)to detect methylation sequential changes in CpG island of its promoter region,to elucidate the interaction between the both.Result With prolongation of time during Islet-1 inducing C3H10T1/2 to differentiate specifically into cardiomyocytes,the expression of the GATA4 gene increased(P<0.05),the level of histone H3K9 acetylation raised(P<0.05),while the DNA methylation level decreased in the second CpG site of its promoter(P<0.05).Conclusion During the Islet-1 promotes cells into cardiac-specific differentiation process,it is indicated that histone acetylation and DNA methylation exist mutual antagonism in regulation of the process of early myocardial specific transcription factor GATA4 expression,which promoted its sequential expression.

Key words: Islet-1, histone acetylation, DNA methylation, mesenchymal stem cells, cardiomyocyte