关键词: 关键词：超保守区域, nPTB, 神经发育, 长非编码RNA

Abstract: Objective Identification of T-uc.33, which is an ultra-conserved lncRNA locates in the genomic loci of nPTB, and exploration of its function during the neural development in mouse. Method Bioinformatics analysis was used to operate the data mining of 481 ultra-conserved regions (UCRs) and identified the T-uc.33 which located in the genomic loci of nPTB using experimental test; RACE was used to obtain the full length of T-uc.33; quantitative real-time PCR was performed to observe the expression pattern of T-uc.33 and nPTB during the developmental stages of mouse brain as well as the differentiation process of neural stem cell; Loss-of-function strategy was used to study the regulatory role of T-uc.33. Results A bona fide ultra-conserved lncRNA named T-uc.33 whose length was 807nt was screened and identified. The expression level of T-uc.33 was significantly positively correlated with nPTB and both of them shared the similar expression pattern during mouse brain development or the differentiation of NSC. Knocking down the expression of T-uc.33 could decrease the abundance of nPTB. Conclusion T-uc.33 is the ultra-conserved lncRNA which locates the genomic loci of nPTB and positively regulates the expression of it. T-uc.33 potentially plays the important role in mouse brain development and NSC differentiation.

Key words: Key words: Ultra-Conserved Regions (UCRs), nPTB, neural development, long non-coding RNAs (lncRNAs)