关键词: 双酚A, 脂质沉积, 内质网应激

Abstract: Objective To investigate the role of endoplasmic reticulum stress（ERS）in hepatic lipid deposition induced by bisphenol A（BPA）. Methods Male CD1 mice were randomly divided into two groups：control group （n = 10）and BPA group（n = 10）. Mice fed with BPA [500μg/(kg?d)] in BPA group for 8 weeks. The content of hepatic triglyceride（TG）was measured by enzymic assay kit and oil red O staining was used to determine the hepatic lipid accumulation. The mRNA levels of fatty acid synthase（FAS）and fatty acid transport protein 1（FATP1）were determined by RT-PCR. Western blot was used to detect the protein expression levels of ERS associated proteins（Bip、p-IRE1α/IRE1α、p-PERK/PERK、p-eIF2α/eIF2α and SREBP-1c）. Results Compared with control group ，the level of TG was higher in BPA group（P < 0.05）. Oil red O staining also revealed that BPA promoted hepatic lipid accumulation. The mRNA levels of FAS and FATP1 were higher in BPA group than those in control group（P < 0.05）. The protein expression levels of Bip、p-IRE1α/IRE1α、p-PERK/PERK、p-eIF2α/eIF2α and SREBP-1c in BPA group were also significantly higher than control group （P < 0.05）. Conclusion ERS could be involved in hepatic lipid deposition of mice induced by BPA.

Key words: bisphenol A, lipid deposition, endoplasmic reticulum stress