与小脑发育相关的一种长非编码RNA Gm2694的鉴定

基础医学与临床 ›› 2016, Vol. 36 ›› Issue (4): 492-497.

与小脑发育相关的一种长非编码RNA Gm2694的鉴定

刘春英¹,张伟龙²,牛亚梅¹,佟伟民¹

1. 中国医学科学院基础医学研究所北京协和医学院基础学院
2. 北京协和医学院中国医学科学院肿瘤医院

收稿日期:2015-12-11 修回日期:2016-01-13 出版日期:2016-04-05 发布日期:2016-03-29
通讯作者: 佟伟民 E-mail:wmtong@ibms.pumc.edu.cn
基金资助:
国家自然科学基金

Characterization of a novel lncRNA Gm2694 specifically expressed in mice cerebellum

Received:2015-12-11 Revised:2016-01-13 Online:2016-04-05 Published:2016-03-29
Contact: Wei-min TONG E-mail:wmtong@ibms.pumc.edu.cn

摘要/Abstract

摘要： 目的鉴定与小鼠小脑发育相关的长非编码RNA，并验证其各剪接变异体的存在。方法用芯片技术分析小鼠小脑发育不同阶段RNA的表达差异；用实时定量PCR技术对芯片结果加以验证；针对目的RNA，验证其各剪接变异体的存在及在小鼠小脑中的表达量。结果 1)长非编码RNA Gm2694在小鼠小脑发育过程中呈动态变化趋势；2)其在小鼠小脑中特异性表达；3) 在小鼠小脑中检测到Gm2694有8种表达水平各不相同的剪接变异体，其中除Ensembl数据库预测的7个之外，发现了1个新的表达于小脑的剪接变异体。结论 Gm2694是一种在小鼠小脑特异性表达的长非编码RNA，存在8种表达量各不相同的剪接变异体。

关键词: 长非编码RNA, Gm2694, 小鼠小脑发育, 剪接变异体

Abstract: Objective To identify novel lncRNAs involved in cerebellar neurogenesis and validate the expression of related splice variants. Methods Microarray analysis of infant (P7) and adult (P28) mice cerebellar RNA was performed to discover novel lncRNAs involved in mice cerebellum neurogenesis. RT-qPCR was then performed to validate the expression of candidate lncRNAs and splice variants. Results A novel lncRNA, Gm2694, was found to be specifically expressed in mice cerebellum in a developmentally regulated manner. In total 8 kinds of splice variants has been detected in cerebellum with various expression levels: besides 7 variants as predicted in the Ensembl databases, another novel variant was identified in mice cerebellum by RACE cDNA amplification. Conclusion Temporal spatial analysis reveals that Gm2694 is a novel lncRNA possibly involved in mice cerebellum neurogenesis.

Key words: long non-coding RNA, Gm2694, mice cerebellar neurogenesis, splice variant

中图分类号:

刘春英张伟龙牛亚梅佟伟民. 与小脑发育相关的一种长非编码RNA Gm2694的鉴定[J]. 基础医学与临床, 2016, 36(4): 492-497.