关键词: Artemis, 人肝癌细胞BEL-7402/5FU, 丝裂霉素C, DNA损伤

Abstract: Objective To explore the cellular effects of shArtemis on DNA damage of human hepatic carcinoma cell line BEL-7402/5FU in vitro.Methods The experiment were divided into control group，Lipofectamine2000 group，contol plasmid group, shArtemis group;Human hepatic carcinoma cell line BEL-7402/5FU were treated with 0.625,1.25,2.5.5.0 and 10.0μg/mL mitomycin C for 24 and 48 huors.BEL-7402/5FU cell activity examined by MTT and observed the expression of phosphorylated histone2AX (γ-H2AX ) by Western blot.After 48h transfected shArtemis interference plasmid , Western blot detected the quantity of Artemis and γ-H2AX ;Commet assy detect the exent of DNA damage.Result The Determined by MTT show that after 48 h respectively treated within 0.625, 1.25, 2.5, 5.0 and 10.0 μg/mL Mitomycin C, cell activity of liver cancer happen to have significantly decreased.In addition, after 48h by incubating with Mitomycin C,the expression of γ-H2AX increased in a dose-dependent manner;The the expression of γ-H2AX increased following inhibitated Artemis. Moreover the tailing DNA of shArtemis experimental group increased than in the control group.Conclusion mitomycin C can induced DNA damage in human hepatic carcinoma cell line BEL-7402/5FU; shArtemis prmotes DNA damage which induced by mitomycin C.

Key words: Artemis, Human hepatic carcinoma cell line BEL-7402/5FU, Mitomycin C , DNA damage