桂皮酸抑制舌鳞癌细胞Tca8113的增殖

基础医学与临床 ›› 2016, Vol. 36 ›› Issue (2): 237-242.

桂皮酸抑制舌鳞癌细胞Tca8113的增殖

凌佳璞¹,王雨坤²,马义丽¹,石昌荣¹,左晓娜¹,黄健¹

1. 桂林医学院
2. 广西大学

收稿日期:2015-07-08 修回日期:2015-11-10 出版日期:2016-02-05 发布日期:2016-01-21
通讯作者: 黄健 E-mail:huangjian@glmc.edu.cn
基金资助:
桂林市科学研究与技术开发计划项目;广西自然科学基金项目

Proliferation inhibition of Tca8113 cell induced by cinnamic acid

Received:2015-07-08 Revised:2015-11-10 Online:2016-02-05 Published:2016-01-21
Contact: Jian HUANG E-mail:huangjian@glmc.edu.cn

摘要/Abstract

摘要： 目的探讨桂皮酸（CINN）对舌鳞癌Tca8113细胞增殖的影响及机制。方法舌鳞癌细胞Tca8113经CINN处理后，倒置显微镜下观察细胞增殖和形态； MTT法检测细胞增殖；流式细胞术检测细胞周期；免疫组化检测KLF6、Bcl-2、p53、cyclin D1、c-Jun及PTEN的蛋白表达。结果 CINN呈时间-剂量依赖性显著抑制Tca8113细胞增殖(p<0.05)；可见典型的Tca8113细胞分化的形态学特征；CINN处理后，G1.G2期细胞显著减少，S期细胞显著增加，细胞明显被阻滞于S期（p<0.05）；CINN处理后KLF6和p53的蛋白表达上调（p<0.05），Bcl-2、cyclin D1及c-Jun的蛋白表达下调（p<0.05）。结论 CINN可抑制舌鳞癌细胞Tca8113的增殖并将其阻止于S期，同时诱导细胞凋亡。

关键词: 舌癌细胞Tca8113, 增殖抑制, 桂皮酸, KLF6

Abstract: Objective To investigate the effect of cinnamic acid on the proliferation of tongue squamous cell carcinoma cell line Tca8113. Methods After treated with cinnamie acid，the morphological changes of Tca8113 were observed under inverted microscope. The proliferation inhibition of Tca8113 cell was detected by MTT method. The cell cycle of Tca8113 was analyzed by flow cytometry. The protein levels of KLF6,Bcl-2,p53,cyclin D1,c-Jun and PTEN were measured by the method of immunohistochemistry. Results It was showed that the proliferation of Tca8113 cells can be inhibited significantly by cinnnamic acid in vitro(p<0.05) ,and the inhibition is time and dose dependent； Typical feature of induced differentiation of Tca8113 cells was observed. After treated with cinnamic acid,the cell number in G1 and G2 phase was decreased significantly while the number in S phase was increased significantly.Tca8113 cells were significantly arrested in S phase (p<0.05) . The protein levels of KLF6 and p53 were increased(p<0.05) whereas the protein levels of Bcl-2, cyclin D1 and c-Jun decreased significantly(p<0.05). Conclusion Cinnamic acid inhibits the proliferation of Tca8113 cell by arresting the cell cycle in S phase,and then may induce TCA 8113 cell to apoptosis finally.

Key words: Tca8113, proliferation inhibition, cinnnamic acid, KLF6

中图分类号:

R73-36

凌佳璞王雨坤马义丽石昌荣左晓娜黄健. 桂皮酸抑制舌鳞癌细胞Tca8113的增殖[J]. 基础医学与临床, 2016, 36(2): 237-242.

[1]	李博王元刚李娟汤海峰程光. 白头翁皂苷—D诱导U87MG细胞凋亡[J]. 基础医学与临床, 2014, 34(5): 654-660.
[2]	宋丽华齐力王敏迟玉涛许小敏. 抗菌肽P18抑制喉癌细胞Hep-2增殖[J]. 基础医学与临床, 2013, 33(9): 1209-1211.
[3]	葛正行李波周洵李常. rHSG基因调控COPD大鼠气道成纤维细胞增殖凋亡[J]. 基础医学与临床, 2013, 33(10): 1235-1241.