关键词: 关键词：慢性阻塞性肺疾病, HSG基因, Wnt/β-catenin信号通路, 气道重建

Abstract: Objective Research the relationship between HSG and Wnt/β-Catenin signal transduction pathway in airway remodeling in COPD,try to elaborate HSG gene regulation mechanism of airway remodeling.Methods rats were divided into normal control group and COPD model group.COPD model group were intratracheal injection of LPS plus smoked methods.Used HE staining to identify COPD model.Determination of the total area of the airway wall/airway basement membrane perimeter(WAt/Pbm)evalution of airway remodeling situation.Cultured the rat airway primary fibroblasts cells in vitro by tissue blocking adhering method.Realtime-PCR were used to detect the HSG and β-catenin mRNA expression in fibroblasts.Used Western blotting assay and ELISA for HSG,β-catenin、GSK-3β,TGF-β1,MMP-9 in small airway fibroblasts.Explored the correlation analysis of HSG,β-catenin with TGF-β1,MMP-9.Results Model group rats small airway remodeling was significantly.COPD model group airway fibroblasts HSG mRNA,protein expression were lower than the normal group. mRNA,protein expression of β-catenin were higher than normal group.Expression of P-GSK-3β，TGF-β1 and MMP-9was also higher than the normal group.HSG mRNA expression and mRNA expression of β-catenin and TGF-β1, MMP-9 secretion of cytokines were negatively correlated.Conclusion The HSG gene might regulated Wnt/β-catenin signaling pathway,whcih resulted in inhibiting the fibroblasts proliferation.

Key words: Key words:COPD, HSG, Wnt/β-catenin signaling pathway, Airway remodeling