关键词: [关键词] 髓母细胞瘤, 原花青素, 细胞凋亡

Abstract: Objective To study the effects of proliferation and apoptosis of human medulloblastoma cell line Daoy induced by proanthocyanidins (PC), furthermore to explore the molecular mechanism. Methods Human medulloblastoma cell line Daoy were treated by PC at different concentrations(0.025,0.05,0.1,0.2,0.4 mmol/L) for 24,48 and 72 h,the inhibition rate of tumor cells were determined by CCK-8 assay. Then, the apoptosis of which were evaluated by Hoechst33258 fluorochrome staining and flow cytometry via Annexin-V/PI labeling method. The protein expressions of Akt,p-Akt,NF-κB were detected by Western blot analysis. Results PC inhibited the proliferation of the Daoy cells in time and dose-dependent manner. After treatment for 72 h,the IC50 of PC was 0.25 mmol/L. We can see many apoptotic cells that the nucleus were condensed, and flow cytometry found that the apoptotic rates of Daoy cells treated by 0.2 mmol/L PC for 24,48,72 h were 21.16%±2.49％,36.02%±0.71％,63.79%±1.47％,respectively.The results showed that the total Akt protein had not been changed, but the p-Akt and NF-κB protein expressions were both down-regulated obviously. Conclusion PC inhibited the proliferation of human medulloblastoma cells and induced their apoptosis, and the molecular mechanism was related with down regulation of p-Akt and NF-κB protein expressions in vitro.

Key words: [Key word] medulloblastoma, proanthocyanidin, apoptosis