关键词: 葡萄糖转运体, 低氧诱导因子-1, 脂多糖, SD大鼠

Abstract: Objective Characterization of glucose transporter (GLUT) family and potential role of HIF-1? in lipopolysacchride (LPS)-induced endotoxemic rats was investigated, and this information helps to understand GLUT family in endotoxemic rats. Methods SD rats were treated with a single dose injection (i.p.) of LPS (2 mg/kg), and rats in control group were given equal amount of NaCl (0.9%) (n=6). The body temperature was detected after LPS treatment for 0 ~ 24 h. IL-1? release was evaluated by ELISA assay. GLUT mRNA expression was determined by RT-PCT assay. HIF-1? and GLUT1 protein expression were examined. Results LPS increased rats’ body temperature and IL-1? release in serum（P<0.05）. GLUT1 and GLUT4 expressed in brain, heart and liver. GLUT2 expressed in brain and liver. GLUT3 mainly expressed in brain. LPS increased GLUT1 mRNA and protein expression in the brain (P<0.01). HIF-1? kept stability in the brain of LPS-challenged rats (P<0.001). Conclusion LPS induced GLUT1 overexpression and HIF-1? stability in the brain of the endotoxemic rats. It indicated that HIF-1?-induced GLUT1 upregulation might be a potential way to regulate glucose metabolism in LPS-challenged rats.

Key words: glucose transporter, hypoxia-inducible factor-1, lipopolysacchride, SD rats