关键词: 肠缺血再灌注, 限制性输液, 开放性输液, 小肠细胞凋亡

Abstract: Object To investigate the effect of different transfusion protocals (restrictive fluid and liberal fluid) on intestinal apoptosis in rabbit intestinal ischemia-reperfusion injury （IRI）. Methods Seventy male rabbits were randomly allocated into the three groups, normal control group (NCG, n=10), intestinal IRI restrictive fluid group (RFG, n=30), intestinal IRI liberal fluid group (LFG, n=30), 24, 48 and 72h after intestine IRI separately rabbits were killed (n=10). Intestinal were cut to detect intestinal apoptosis by TUNEL and the expression of Bcl-2、Bax by q-RT-PCR were detected. Results The number of intestinal apoptosis after intestinal IRI in restrictive fluid group significantly lower than that in liberal fluid group（P<0.01）. Anti-apoptosis gene Bcl-2 in RFG were higher than that in LFG（P<0.01）, while pro-apoptosis gene Bax in RFG was less than LFG（P<0.01）. Conclusion The effect restrictive fluid on intestine was mild than LFG, which could be due to apoptosis is much less.

Key words: intestinal ischemia-reperfusion injury, restrictive fluid, liberal fluid, intestinal apoptosis