关键词: 胃癌, MGC-803细胞, E2F-1，过表达

Abstract: Objective To study the molecular mechanisms of human gastric cancer cell line MGC-803 proliferation suppressed by overexpression of E2F-1 . Methods The experimental group(LV-E2F-1-GFP group ),gastric cancer MGC-803 cells were transfected with the recombinant lentivirirus vector (LV-E2F-1-GFP) ,the negative control group were transfected with the negative control lentiviral vector (LV- GFP),the blank control group was untreated. The expression of Skp2, Bax, Bcl-2 and Survivin were detected by semi-quantitative PT-PCR and Western blot method . Results Compared with LV- GFP group and blank control group,the mRNA levels of Skp2, Bcl-2 and Survivin in the LV-E2F-1-GFP group were reduced(P<0.05),the protein levels of Skp2, Bcl-2 and Survivin in the LV-E2F-1-GFP group were reduced(P<0.05), the mRNA level of Bax and the protein level of Bax were up regulated (P<0.05). Conclusion The proliferation of human gastric cancer MGC-803 cells was inhibited by overexpression of E2F-1 mediated by lentivirus, the effect may be related with down-regulation of Skp2,Survivin,Bcl-2 expression and up-regulation of Bax expression.

Key words: Gastric cancer , MGC-803 cells , E2F-1, overexpression