基础医学与临床 ›› 2013, Vol. 33 ›› Issue (10): 1259-1263.

• 研究论文 • 上一篇    下一篇

中介素减轻大鼠高肺血流性肺血管结构重构

庞璐璐,齐建光,高扬,金红芳,杜军保   

  1. 北京大学第一医院
  • 收稿日期:2012-12-21 修回日期:2013-03-27 出版日期:2013-10-05 发布日期:2013-09-25
  • 通讯作者: 齐建光 E-mail:qjg2006@126.com
  • 基金资助:
    自发性高血压大鼠心、脑、肾和肠系膜微动脉缝隙连接特性的比较研究》国家自然科学基金(国家自然科学基金)

Intermedin alleviates the development of pulmonary vascular structural remodeling induced by high pulmonary blood flow in rats

  • Received:2012-12-21 Revised:2013-03-27 Online:2013-10-05 Published:2013-09-25
  • Contact: Jian-Guang QI E-mail:qjg2006@126.com

摘要: 目的 研究中介素(IMD)对大鼠高肺血流性肺血管结构重构的调节作用及其机制。方法 雄性SD大鼠21只,随机分为对照组(n=7),分流组(n=8)和分流+IMD组(n=6)。对后两组大鼠行腹主动脉-下腔静脉分流术。8周后,对分流+IMD组大鼠,皮下埋微量渗透泵持续予IMD 1.5 μg/(kg?h)。继续饲养2周后测定动脉平均压(mPAP)和右心室/(左心室+室间隔)重量比值,检测肺血管形态学变化,硝酸酶还原法测定血清和肺组织匀浆一氧化氮(NO)含量,Western blot方法测定肺组织内皮型一氧化氮合酶(eNOS)含量。结果 与对照组相比,分流组大鼠mPAP、右心室肥厚程度、肺小血管肌化程度以及肺中、小动脉相对中膜厚度均明显增加,血清和肺组织匀浆NO含量明显降低,肺组织eNOS蛋白含量降低。IMD使肺动脉压力明显回降,右心室肥厚程度减轻,肺血管结构改变缓解,血清和肺组织匀浆NO水平以及肺组织eNOS蛋白含量均明显升高。结论 IMD可能通过eNOS/NO途径,减轻高肺血流性肺动脉高压和肺血管结构重构的形成。

关键词: 肺动脉高压, 高肺血流, 中介素, 一氧化氮

Abstract: Objective To explore the regulating effect of intermedin (IMD) on the pulmonary vascular structural remodeling and pulmonary hypertension induced by high pulmonary blood flow in rats and its mechanism. Methods Twenty-one male SD rats were randomly divided into control group (n=7), shunt group (n=8) and shunt with IMD group (n=6) . Abdominal aorta and inferior vena cava shunting was produced in rats of the latter two groups. After 8 weeks, IMD [1.5 μg/(kg?h)] was administered into rats of shunt with IMD group subcutaneously by mini-osmotic pump for 2 weeks. Mean pulmonary artery pressure (mPAP) and the ratio of right ventricular mass to left ventricular plus septal mass [RV/(LV+SP)] was evaluated. The pulmonary vascular micro-morphologic changes of rats were observed. The content of NO in the serum and lung tissue homogenate was detected by nitric acid enzyme reduction method. The expression of eNOS protein in the lung tissue was detected by Western blotting analysis. Result Compared with those of control group, mPAP, RV/(LV+SP), the muscularization of small pulmonary vessels and relative medial thickness of pulmonary artery in rats of shunt group were all significantly increased. Meanwhile, the content of NO in the serum and pulmonary tissue homogenate and the expression of eNOS protein in rats of shunt group were all significantly decreased compared with those of control group. However, IMD significantly decreased the mPAP and RV/(LV+SP), alleviated the changes of pulmonary vascular micro-structure, with the elevation of the content of NO in the serum and pulmonary tissue homogenate and the expression of pulmonary eNOS protein in shunting rats. Conclusion IMD alleviates the development of pulmonary hypertension and pulmonary vascular structural remodeling induced by high pulmonary blood flow, through eNOS/NO path way.

Key words: pulmonary hypertension, high pulmonary blood flow, intermedin, nitric oxide

中图分类号: