FK506对NIT-1细胞增殖和胰岛素分泌的影响

基础医学与临床 ›› 2013, Vol. 33 ›› Issue (9): 1191-1194.

FK506对NIT-1细胞增殖和胰岛素分泌的影响

黄小芳¹,丁香²,张应辉¹

1. 中国人民解放军第一八一医院药剂科
2. 中国人民解放军第一八一医院

收稿日期:2012-07-03 修回日期:2012-12-06 出版日期:2013-09-05 发布日期:2013-08-28
通讯作者: 张应辉 E-mail:1135038142@qq.com

Effect of FK506 on NIT-1 cells proliferation and insulin secretion

Received:2012-07-03 Revised:2012-12-06 Online:2013-09-05 Published:2013-08-28

摘要/Abstract

摘要： 目的探讨他克莫司（FK506）对NOD鼠胰岛β细胞株（NIT-1）增殖、凋亡和胰岛素分泌的影响。方法用MTT法、流式细胞术检测NIT-1细胞增殖；放射免疫法、Real-time PCR检测NIT-1细胞胰岛素分泌和胰岛素分化相关基因PDX-1、GLUT2和GK mRNA的表达。结果 FK506（20μg/L）抑制NIT-1细胞增殖（P<0.05），诱导细胞凋亡（P<0.05）；FK506（10 μg/L）抑制NIT-1细胞释放胰岛素（P<0.05），胰岛素分泌相关基因PDX-1（胰岛素促进因子-1）、GLUT2（葡萄糖转运载体2） mRNA表达下调（P<0.05）。结论 FK506（10μg/L）可抑制NIT-1的增殖，诱导凋亡，可通过下调PDX-1和GLUT2 mRNA表达，抑制NIT-1胰岛素分泌。

关键词: 关键词：NIT-1细胞, 增殖及凋亡, 胰岛素分泌, PDX-1, GLUT2

Abstract: Objective To investigate the effects of FK506 on cell proliferation of pancreatic NIT-1 beta-cell and insulin secretion function. Methods Effect of FK506 on proliferation and apoptotic were mensured by MTT-assay, Flowcytometry; Insulin secretion was investigated by Radioimmunoassay and Real-time PCR. Results FK506 (20μg?L-1) could inhibit the proliferation of NIT-1 cells, and could induce apoptosis; FK506(10μg?L-1) could inhibit NIT-1 cell insulin release, The mRNA expression of PDX-1 and GLUT2 were down-regulated. Conclusion FK506 inhibit NIT-1 cells insulin secretion, the mechanism maybe related to the down-regulation of. PDX-1 and GLUT2.

Key words: Key words: NIT-1 beta-cell, apoptosis, secretion level of insulin, PDX-1, GLUT2

黄小芳丁香张应辉. FK506对NIT-1细胞增殖和胰岛素分泌的影响[J]. 基础医学与临床, 2013, 33(9): 1191-1194.