关键词: [关键词] 高甘油三酯血症, 脂质肾毒性, IV型胶原, α-平滑肌动蛋白

Abstract: [Abstract] Objective To investigate the effect of high fructose-induced hypertriglyceridmia on kidney of healthy Wistar rats. Methods Thirty-two healthy male Wistar rats were randomly divided into two groups (n =16): normal control group and high fructose group. Rats were sacrificed by the end of 8 and 16 weeks. BUN, Scr, fast glucose ,lipid concentration, urinary microalbumin were observed. Triglyceride of renal cortices were detected. The pathological changes were examined by PAS staining. IV-type collage and α-SMA protein were examined by immunohistochemistry staining. The electron microscope sections were made to measure GBM width Results By the end of 8 and 16 weeks, compared with the control group, high fructose group showed elevated triglyceride (1.65+0.86)mmol/L、（2.13+0.87）mmol/L(both P<0.05 ), and very low density lipoprotein chloesterol(0.75+0.39)mmol/L、(0.97+0.40)mmol/L(both P<0.05 ) in blood. Renal cortices were both significantly increased(7.21+2.20)mg/g、(7.92+3.05)mg/g (both P<0.05). The urinary microalbumin significantly increased too(63.00+12.00)μg、(150.00+48.00) μg (both P<0.05). The pathological changes were serious in the high fructose group. The expression of IV-type collagen and α-SMA protein were up-regulated. Electron microscope revealed GBM obviously thickened in high fructose group. Conclusion High fructose-induced hypertriglyceridmia may cause lipid nephrotoxicity in Wistar rats.

Key words: [Keywords] hypertriglyceridmia, lipid nephrotoxicity , IV-type collage, α-smooth muscle actin