ANO1在口腔鳞癌中的表达及临床分析

基础医学与临床 ›› 2012, Vol. 32 ›› Issue (10): 1175-1178.

ANO1在口腔鳞癌中的表达及临床分析

李雅冬¹,张劲松²,杨凯²,张福军²,陈睿²,洪苏玲³

1. 重庆医科大学附属第一医院口腔科
2. 重庆医科大学附属第一医院
3. 重庆医科大学附属第一医院耳鼻咽喉科

收稿日期:2011-12-12 修回日期:2012-03-02 出版日期:2012-10-05 发布日期:2012-09-28
通讯作者: 李雅冬 E-mail:llxxyydd2006@sina.com
基金资助:
重庆市卫生局2011 年医学科研计划项目（面上项目）资助

Clinical significance and expression of ANO1 in oral squamous cell carcinoma

Received:2011-12-12 Revised:2012-03-02 Online:2012-10-05 Published:2012-09-28
Contact: li yadong E-mail:llxxyydd2006@sina.com
Supported by:
chongqing municipal health brueau medical science technique project

摘要/Abstract

摘要： 目的探讨ANO1基因及蛋白在口腔鳞癌中的表达及其临床意义。方法应用免疫组化SP法及Northern blot检测81例口腔鳞癌组织及相应正常组织的ANO1基因及蛋白的表达进行检测，并结合临床病理资料和基因蛋白表达特征对比作差异显著性检验及相关分析。利用western blot检测ANO1在多株鳞癌细胞株的表达。结果 ANO1在口腔鳞癌组织中的阳性表达明显高于正常组织，有显著性差异( P <0.05)；有淋巴结转移的口腔鳞癌组织ANO1阳性表达高于无转移的口腔鳞癌组织。有显著性差异( P <0.05)；随着口腔鳞癌临床分期的升高，ANO1的阳性表达率升高(P＜0.05)；而ANO1的阳性表达率与病理分级,年龄和性别暂无关(P＞0.05)。Hep-2的内源性ANO1表达最低，而SCC-25细胞株的内源性ANO1表达最高。结论 ANO1可能在口腔鳞癌的发生和进展过程中起到重要作用。

关键词: 口腔鳞癌, ANO1基因, Northern blot

Abstract: Objective To study the clinical significance of ANO1 mRNA and protein in oral squamous cell carcinoma (OSCC) and to find the biological foundation of making a proper treatment for OSCC. Methods The specimens of 81 patients with OSCC were examined by using SP immunohistochemistry and northern blot.The expression of ANO1 protein and clinical and pathological feature were determined and analyzed.The expression of ANO1 protein in head and neck cancer cell lines were examined by western blot.Results ANO1 mRNA and protein expression were significantly higher in OSCC than those in normal oral tissue(P＜0.05), and showed significant correlation with clinical stage of OSCC (P＜0.05) and lymph node metastasis (P<0.05). No statistical significance was observed in of the ANO1 expression in histological grades and patient sex, age (P＞0.05). The lowest endogenous ANO1 was expressed in Hep-2 cell line. The most endogenous ANO1 was expressed in SCC-25 cell line. Conclusion ANO1 may be involved in the occurrence, metastasis and progress of OSCC. Analysis of the expression characteristics of ANO1 and clinic and pathological features of OSCC may better conduct clinical therapy of OSCC.

Key words: oral cancer, ANO1, Northern blot

李雅冬张劲松杨凯张福军陈睿洪苏玲. ANO1在口腔鳞癌中的表达及临床分析[J]. 基础医学与临床, 2012, 32(10): 1175-1178.