关键词: 骨髓间充质干细胞, 重组腺病毒-绿色荧光蛋白, 移植, 子宫修复

Abstract: Abstract Objective To investigate growth and differentiation of adenovirus-green fluorescent protein labelled bone marrow mesenchymal stem cells (BM-MSCs) in uterus incision, and discuss its potential regeneration repair effect on uterine incision in rat. Methods BM-MSCs were separated by density gradient centrifugation and cultured until to the fourth generation, then were transfected and labelled with adenovirus-green fluorescent protein. Fourty female SD rats were divided equally into control and experimental group, followed with the establishment of uterine wound model. After two weeks, adenovirus-green labelled BM-MSCs were transplanted directly into uterus at previous incision, with the control group only received normal saline injection. Then, one month later, CTGF、CD34 and Masson was used to detect the outcome of the transplanted BM-MSCs.Results The transplanted BM-MSCs can growth well and showed some extent of differentiation toward smooth muscle cells. Significant difference existed for CTGF and Masson between experimental and control samples (both P<0.01).Conclusion BM-MSCs transplantation is a promising technique and can be used to promote regeneration repair of uterine incision.

Key words: Bone marrow mesenchymal stem cells, Adenovirus-green fluorescent protein, Transplantation, uterus repair