关键词: 维吾尔族妇女, 宫颈癌, 蛋白质组, 二维液相色谱

Abstract: Objective To analyze the proteomic profile of low abundant plasma proteins in cervical squamous cell carcinoma（CSSC）of Uighur women by using two dimensional liquid phase chromatography, to identify differences of plasma proteome between early and advanced stages of the cancer. Methods Forty-six cases of plasma samples from Uighur women with CSSC were collected，including 26 cases of early stage cancer atⅠ-Ⅱa and 20 cases of advanced stage cancer at Ⅱb-Ⅳ; After preparation of the proteome of low abundant plasma proteins by depletion of high abundant proteins, the proteomic fingerprint features of patients with early stage and advanced stage of cancer were studied by two-dimensional liquid phase chromatography (ProteomeLabTM PF- 2 D) , to establish a distinguishing model of plasma proteome for patients withdifferent stages of cancer. Results A plasma proteome fingerprint model specific to patients with early stage and advanced stage of cancer was established. By setting up the protein content difference of more than two times as a standard for quantitative differences, 5 peaks with ascending and 10 with descending protein content in patients with advanced stage of cancer compared to early stages were found. Conclusion The remarkable difference in low abundant plasma proteomes between early and advanced stages of CSCC in Uighur women may provide an important evidence for the identification of plasma protein markers specific to the cancer progression in the future.

Key words: Uighur women, cervical carcinoma, proteome, 2-D liquid phase chromatography