TRAIL对CIA小鼠脾细胞表达Th1/Th2细胞因子的影响

基础医学与临床 ›› 2011, Vol. 31 ›› Issue (12): 1330-1334.

TRAIL对CIA小鼠脾细胞表达Th1/Th2细胞因子的影响

刁智娟,朱洁卿,史娟,郑德先

中国医学科学院基础医学研究所

收稿日期:2011-10-10 修回日期:2011-10-26 出版日期:2011-12-05 发布日期:2011-12-05
通讯作者: 史娟 E-mail:shijuantt@yahoo.com
基金资助:
自然基金

TRAIL modulates splenocyte Th1/Th2 cytokines profile of CIA mice

Received:2011-10-10 Revised:2011-10-26 Online:2011-12-05 Published:2011-12-05

摘要/Abstract

摘要： 摘要：目的观察肿瘤坏死因子相关凋亡诱导配体（Tumor Necrosis Factor Related Apoptosis Inducing Ligand , TRAIL）对胶原诱导型关节炎（Collagen-induced arthritis, CIA）小鼠的治疗效果，探讨TRAIL治疗类风湿性关节炎（Rheumatoid Arthritis, RA）的可能机制。方法牛Ⅱ型胶原蛋白加弗氏完全佐剂免疫DBA/1J小鼠建立CIA模型，采用重组腺相关病毒AAV-TRAIL关节腔内注射进行治疗。CBA和流式细胞术检测Th1/Th2细胞因子的分泌。结果 AAV-TRAIL可改善CIA小鼠的病情，体外研究其机制发现TRAIL可抑制CIA小鼠的脾细胞分泌Th1型细胞因子，但对Th2型细胞因子没有作用。结论TRAIL对CIA小鼠的治疗作用可能和TRAIL抑制Th1型细胞因子分泌相关。

关键词: 类风湿关节炎, CIA小鼠, TRAIL, Th1细胞因子, Th2细胞因子

Abstract: Abstract：Objective To investigate the effect of TRAIL on CIA mice and its possible mechanism. Methods CIA was induced in DBA/1J mice with bovine type II collagen and Freund’s adjuvant. AAV-TRAIL particles were injected intra-articularly into knee joints of the mice. Th1/Th2 cytokines were detected by CBA method. Results The intra-articular injection of AAV-TRAIL reduced the severity of joint swelling in the CIA mice. And TRAIL inhibited Th1 cytokines release of splenocyte from CIA mice, but did not influence Th2 cytokines release. Conclusion TRAIL inhibited Th1 cytokines release was perhaps involved in anti-arthritis effect of TRAIL.

Key words: Rheumatoid Arthritis (RA), Collagen-induced arthritis (CIA) mice, TRAIL, Th1 cytokine, Th2 cytokine

中图分类号:

R593.22

刁智娟朱洁卿史娟郑德先. TRAIL对CIA小鼠脾细胞表达Th1/Th2细胞因子的影响[J]. 基础医学与临床, 2011, 31(12): 1330-1334.

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