关键词: 内侧颞叶癫痫, 海人酸, C57BL/6小鼠, 海马硬化

Abstract: Objective To establish the kainic acid (KA)-induced mesial temporal lobe epilepsy (TLE) mice model and detect the characteristic changes. Methods Healthy male C57BL/6 mice were randomly divided into control group (non-injected), saline group (35 μL/g) and KA group (12 mg/kg) for intrahippocampal injections. 5 days and 5 weeks after injection,the mice brains were sectioned and stained by hemotoxylin-eosin (H&E) to detect the pathologic changes in the hippocampal area; the expression level of P-S6 protein, the marker of hippocampus mTOR signal pathway, was also determinated to verify the animal model. Results （1）Only KA-treated mice showed wet dog shakes, facial clonus and generalized tonic-clonic convulsions.（2）After injection of KA, neuronal cell loss was prominent in in CA1, CA3 and hilar area of the ipsilateral hippocampus; granule cell in dentate gyrus area dispersed, which are fertures of hippocampus sclerosis.（3）P-S6 expression level increased in both acute (P < 0.05) and chronic phases (P < 0.01) of MTLE models. Conclusion Intrahippocampal injection of KA in C57BL/6 mice could induce behavioural, histopathological changes which are similar to those clinic features of MTLE.

Key words: mesial temporal lobe epilepsy, kainic acid, C57BL/6 mice, hippocampus sclerosis