Pharmacodynamic Study of Polygonatum Sibiricum Polysaccharide and Asiaticoside on Caudal Fin Regeneration of Zebrafish with Type 2 Diabetes
BIAN Jin-huan1a, DU Li-ji2, WANG Zi-li1b, XU Shu-wen2*, GONG Zhu-nan1a,1b*
1a. College of Life Sciences, 1b. School of Food and Pharmaceutical Engineering, Nanjing Normal University, Nanjing 210046, China; 2. Anhui Academy of Science and Technology, Hefei 230088, China
Abstract：OBJECTIVE To explore the effects of polygonatum sibiricum polysaccharide (PSP) and asiaticoside (AS) on the regeneration of caudal fin in type 2 diabetic zebrafish. METHODS First, the safe dosages of the drugs were analyzed, and then a zebrafish type 2 diabetes model was constructed by microinjection of 0.3% streptozotocin (STZ). After the tail of the type 2 diabetic zebrafish was docked, PSP, AS and combined drugs were given, and real time quantitative PCR(qRT-PCR) was used to detect the effects on the expression of bone morphogenetic protein (bmp2b), fibroblast growth factor (fgf20a) and matrix metalloproteinase (mmp13) related to zebrafish tail fin regeneration. RESULTS Three days after STZ injection, the fasting blood glucose of zebrafish was 2.726 4 mg·mL-1, indicating that the modeling was successful. Compared with the model group, the length of caudal fin regeneration increased significantly in the AS 150 μg·mL-1, PSP 75 μg·mL-1, AS 75 μg·mL-1 +PSP 37.5 μg·mL-1 groups. The expression of bmp 2b in the AS 75 μg·mL-1, AS 75 μg·mL-1 + PSP 37.5 μg·mL-1 group was significantly increased, surpassing that of the metformin group. The expression of fgf 20a in the AS 300 μg·mL-1 group was up-regulated to a very significant level (P<0.001), and the expression of mmp13 gene was up-regulated in the administration group, but the effect was not as good as that in the metformin group. CONCLUSION AS, PSP and their combination had the effects of lowering blood sugar and promoting tail fin regeneration in type 2 diabetic zebrafish, and the latter effect may be produced by promoting the gene expression of bmp 2b, fgf 20a, and mmp13. Overall, AS more effectively increased the expression of genes related to zebrafish tail fin regeneration.
边金焕, 杜李继, 王子丽, 许舒雯, 龚祝南. 黄精多糖与积雪草苷对糖尿病斑马鱼尾鳍再生的药效学研究[J]. 中国药学杂志, 2023, 58(7): 592-598.
BIAN Jin-huan, DU Li-ji, WANG Zi-li, XU Shu-wen, GONG Zhu-nan. Pharmacodynamic Study of Polygonatum Sibiricum Polysaccharide and Asiaticoside on Caudal Fin Regeneration of Zebrafish with Type 2 Diabetes. Chinese Pharmaceutical Journal, 2023, 58(7): 592-598.
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2023, Vol. 58 
