Abstract：OBJECTIVE To observe the effects of Panax notoginseng saponins (PNS) on the ratio of macrophage M1 polarization and inflammatory factor TNF-α, and preliminarily explore its relationship with regulating the expression of GAS6/MERK pathway. METHODS The single culture of THP-1-derived macrophages and Transwell co-culture system of THP-1-derived macrophages and HFLS-RA were established respectively. LPS+IFN-γ induced the polarization of macrophage M1. Then the cells were divided into control group and PNS intervention groups (5, 10, 20 μg·mL-1). After adding PNS for 48 h, the optimal concentration of PNS was selected by CCK-8 method. The ratio of macrophage M1 polarization was detected by flow cytometry. The expression levels of TNF-α, MERTK receptor and GAS6 ligand were detected by qPCR. The secretion levels of TNF-α and GAS6 were detected by ELISA. Total protein and phosphorylation of MERTK receptor were detected by Western blot. RESULTS In the single culture of THP-1-derived macrophages, 5-20 μg·mL-1 PNS could significantly inhibit macrophages M1 polarization and up-regulate MERTK expression. In Transwell co-culture system, 5-20 μg·mL-1 PNS could also significantly inhibit M1 polarization and up-regulate the expression of MERTK. Compared with the single culture system, 5 μg·mL-1 PNS could significantly up-regulate the secretion level of GAS6 in HFLS-RA, and the phosphorylation level of MERTK was significantly up-regulated after 5 and 10 μg·mL-1 PNS treatment. CONCLUSION Certain concentrations of PNS can significantly inhibit the ratio of macrophage M1 polarization induced by LPS+IFN-γ and the expression level of inflammatory factor TNF-α, and its mechanism may be related with regulating the expression of GAS6/MERK pathway.
狄昱希, 包宇杰, 刘杨添一, 朱志祺, 周玲玲. 三七总皂苷对巨噬细胞M1极化的影响及机制初探[J]. 中国药学杂志, 2023, 58(7): 576-583.
DI Yu-xi, BAO Yu-jie, LIU Yang-tian-yi, ZHU Zhi-qi, ZHOU Ling-ling. Preliminary Exploration of the Effect of Panax notoginseng Saponins on Macrophage M1 Polarization and Its Mechanism. Chinese Pharmaceutical Journal, 2023, 58(7): 576-583.
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