目的 建立高效,稳定的超高效液相色谱-线性离子阱轨道阱串联质谱联用(UHPLC-LTQ-Orbitrap-MS/MS) 方法,系统、全面地定性分析中药制剂保心宁胶囊中的化学成分组成。为保心宁胶囊质量控制方法的开发,标准的完善以及临床应用提供依据。方法 采用UHPLC-LTQ-Orbitrap-MS/MS对保心宁胶囊中的化学成分进行快速分析。通过UHPLC-LTQ-Orbitrap-MS/MS扫描的到的化合物精确分子量,对照品和多级碎片离子信息,并结合相关文献以及裂解规律,实现对保心宁胶囊的准确定性分析。结果 共鉴定出103种化学成分,包括萜类、皂苷类、酚酸类、黄酮类、有机酸类、香豆素类、苯酞类及姜黄素类等,并对化合物的药材归属进行了分类。结论 本研究建立了对保心宁胶囊中化学成分的快速定性分析的方法,为保心宁胶囊的质量评价指标及药效物质基础研究提供参考。
Abstract
OBJECTIVE To establish an efficient and stable ultra-high performance liquid chromatography linear ion trap orbital trap tandem mass spectrometry (UHPLC-LTQ-ORBITRAP-MS/MS) method for systematic and comprehensive qualitative analysis of chemical components in Baoxinning capsules, and provide a basis for the development of quality control methods, improvement of standards and clinical application of Baoxinning Capsules. METHODS The chemical components in Baoxinning capsules were analyzed by UHPLC-LTQ-ORBITRAP-MS/MS. The accurate molecular weight, reference substance and multistage fragment ion information of the compound were obtained by UHPLC-LTQ-ORBITRAP-MS/MS, combined with relevant literature and cracking law. RESULTS A total of 103 chemical constituents were identified, including terpenoids, saponins, phenolic acids, flavonoids, organic acids, coumarins, phthalides, curcumins and others. CONCLUSION A rapid qualitative analysis method of chemical components in Baoxinning capsules is established,and this study has a certain reference significance for the quality evaluation index and pharmacodynamic material basis of Baoxinning capsule.
关键词
保心宁胶囊 /
超高效液相色谱-线性离子阱轨道阱串联质谱联用 /
萜类 /
皂苷类 /
酚酸类 /
黄酮类 /
苯酞类 /
香豆素类
{{custom_keyword}} /
Key words
Baoxinning capsule /
UHPLC-LTQ-Orbitrap-MS/MS /
terpenoid /
saponin /
phenolic acid /
flavonoid /
phthalide /
coumarin
{{custom_keyword}} /
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
参考文献
[1] HE Y T, LIN Y F, BIAN C Z, et al. Improvement of the Quality Standard of Baoxinning Capsules. Chin J Etho Med Ethnopha(中国民族民间医药杂志), 2019, 28(22):46-49.
[2] ZHANG Y Q. Clinical effect of Baoxinning capsule combined with mexiletine in the treatment of patients with chronic ventricular arrhythmia. Clin Res Pract(临床医学研究与实践), 2021, 6(8):60-61,71.
[3] XU Y R, SHI J, GONG H Q, et al. Protection of Baoxinning Tablets on myocardial ischemia in rats with coronary artery Ligation. Ginseng Res(人参研究杂志), 2018, 30(6):12-14.
[4] ZHAI H Y, SHAN B Y, WANG H Y, et al. Simultaneous determination of six index components in Baoxinning tablets by a single marker. Chin J Pharm Anal(药物分析杂志), 2018, 38(6):973-978.
[5] WANG X Y, GUO S. Determination of Ginsenoside Rg1 in Baoxinning Tablets by HPLC. J Henan Univ Med(河南大学学报), 2017, 36(4):263-264.
[6] ZHAI H Y, WANG H Y, YANG X L, SHAN B Y. Study on HPLC specific chromatogram of Baoxinning tablets and simultaneous determination of its eight index components. Chin J Pharm Anal(药物分析杂志), 2017, 37(9):1633-1639.
[7] ZUO L H, ZHOU L, LI Z L, et al. Study on chemical constituents of Tiandan Tongluo Capsule based on UPLC-Q-Orbitrap HRMS. Chin Tradit Herb Drugs(中草药), 2018, 49(5):1031-1040.
[8] QIN Y, ZHAO X J, GUO P M, et al. Analysis of bioactive compounds in sour orange fruit based on UPLC-QTOFMS/MS . Food Ferment Ind(食品与发酵工业): 1-12 . https://doi.org/10.13995/j.cnki.11-1802/ts.28237.
[9] YANG X X, LIU C X, YAN C Y, et al. Analysis of chemical constituents of Huoxue Jiedu Decoction extract by UPLCQ-TOF/HRMSE. Drug Eval Res(药物评价研究杂志), 2020, 43(11):2207-2216.
[10] LIU L, DUAN J A, TANG Y P. Study on chemical component and synergetic effect of concerted application of Chinese angelica and safflower by using UHPLC-Q-TOF-MS . Chin J Tradit Chin Med Pharm(中华中医药杂志), 2017, 32(3):996-1000.
[11] ZENG Z, XIE R Q, ZHANG T, et al. The Regular Patterns of Mass Spectrometry of Lactons from Ligusticum chuanxiong Hort. J Chin Mass Spectr Soc(质谱学报), 2011, 32(5):293-300.
[12] ZHANG J H, WU W D, LIU J T, et al. Rapid analysis of chemical constituents of Huoxue Zhitong Capsules based on UPLC-Q-TOF/MS. Chin Tradit Herb Drugs(中草药), 2020, 51(12):3139-3146.
[13] LOU T Y, MA B B, LIANG Y Y, et al. Analysis and identification of metabolites of cryptotanshinone in rats by UHPLC-Q-Exactive mass spectrometer. J Chin Mass Spectr Soc(质谱学报), 2020, 41(4):307-320.
[14] CHEN J H, ZHANG Y X, LIU M H, et al. Chemical profiling of Danshen water extract by UPLC-Q-TOF-MS /MS. J Guangdong Pharm Univ(广东药科大学学报), 2020, 36(1):1-9.
[15] QI X X, DONG Y, SHAN C X, et al. Analysis of the main components in Herbal Pair: Astragali Radix and Salviae Miltiorrhizae by UFLC-Q-TOF/MS. J Nanjing Univ Tradit Chin Med(南京中医药大学学报), 2017, 33(1):93-96,103.
[16] TU Y, SUN L N, DONG Z Y, et al. Study on chemical constituents differences of Saliviae miltiorrhizae Radix et Rhizoma from different producing areas based on UPLC-QTOF-MS technology. J Chin Med Mater(中药材), 2021, 44(6):1337-1342.
[17] CHEN Y Q, FAN X S, ZHU Z H, et al. Chemical constituents of Shuangshen Pingfei Granules by UPLC-ESI-Q-TOF-MS/MS. Chin Tradit Herb Drugs(中草药), 2020, 51(2):321-329.
[18] ZHANG C, ZHANG C J, QIU M Y, et al. Quick feature analyzation of chemical constituents of Radix et Rhizoma salviae miltiorrhizae by UPLC -LTQ orbitrap MS. Acta Chin Med Pharm(中医药学报), 2018, 46(3):14-21.
[19] YAN X J, ZHENG B W, ZHANG Y D, et al. Changes of chemical components in raw products and characteristic processed products with porcine cardiac blood of salviae miltiorrhizae Radix et Rhizoma from menghe medical school by UPLC-Q-TOF /MS. Chin J Exp Tradit Med Form(中国实验方剂学杂志), 2019, 25(9):109-116.
[20] ZHAO J, QIN Z X, PENG B, et al. Fragmentation pathway of Ginsenosides in Panaxnotoginseng using electrospray Ionization-quadrupole/time-of-flight mass spectrometer. J Chin Mass Spectr Soc(质谱学报), 2017, 38(1):97-108.
[21] LI X F, ZHANG S Q, LIU J B, et al. Analysis and identification of main components of Wenxin Granules based on UPLC/Q-TOF MS/MS. Tianjin J Tradit Chin Med(天津中医药), 2020, 37(8):943-948.
[22] GUI Q, JIE Z. Simultaneous determination of eight components in Angelica sinensis based on UHPLC-ESI-MS/MS method for quality evaluation. Biomed Chromatogr, 2018, 33: e4326.
[23] JIANG H G, ZHAO X K, LIN W Y, et al. Qualitative analysis of multiple cumarins in angelicae sinensis radix based on HPLC-Q-TOF-MS/MS. Biomed Chromatogr, 2021, 44(6). Doi:10.1002/bmc.4326
[24] WANG X G, YANG H J, WANG S S, et al. Fragmentation pathways of five furocoumarine using line ion trap with orbitrapmass spectrometry. China J Chin Mater Med(中国中药杂志), 2015, 40(7):1334-1341.
[25] LI M Q, ZHANG X J, HU X L, et al. Fragmentation pathway of gingerol using electrospray Ionization with quadrupole time-of-flight mass spectrometry. J China Mass Spectr Soc(质谱学报), 2021, 42(3):218-227.
[26] WANG L, FANG L, ZHAO HQ, et al. Analysis of gingerol-related compounds in fresh ginger by HPLC-ESI-Q-TOF-MS /MS. China J Chin Mater Med(中国中药杂志), 2011, 36(24):3467-3471.
[27] LIANG N, SANG Y S, ZHOY Y, et al. Analysis of gingerol compounds in rhizoma zingiberis by HPLC-ESI-Q-TOF-MS /MS. Sci Technol Food Ind(食品工业科技), 2018, 39(9):252-256.
[28] YU G H, YANG H J, LI JF, et al. Analysis of chemical constituents from Citrus aurantium by UHPLC-LTQ-Orbitrap-MS /MS. China J Chin Mater Med(中国中药杂志), 2016, 41(18):3371-3378.
[29] MAO Y N, WU W Q, KANG Y, et al. Rapid analysis of chemical components in Zizyphi Spinosae Semen by UHPLC-LTQ-Orbitrap-MS. China J Chin Mater Med(中国中药杂志), 2018, 43(24):4884-4891.
{{custom_fnGroup.title_cn}}
脚注
{{custom_fn.content}}
基金
浙江省科技计划项目-药食同源功能健康食品开发-药食同源功能组分膜法制备及其功能健康食品全利用开发项目资助(2021C02019)
{{custom_fund}}
PDF(2030 KB)
