Rapid Analysis of Chemical Components in Baoxinning Capsules Based on UHPLC-LTQ-Orbitrap-MS/MS Technology
DU Ya-qiang1, YANG Huan2,3, TAN Chun-mei2,3, CHEN Bi-lian2,3*, LUO Lei2,3*
1. College of Pharmaceutical Science, Zhejiang Chinese Medical University, Hangzhou 310053, China; 2. Zhejiang Institute for Food and Drug Control, Hangzhou 310052, China; 3. NMPA Key Laboratory for Quality Evaluation of Traditional Chinese Medicine(Traditional Chinese Patent Medicine), Hangzhou 310052, China
Abstract:OBJECTIVE To establish an efficient and stable ultra-high performance liquid chromatography linear ion trap orbital trap tandem mass spectrometry (UHPLC-LTQ-ORBITRAP-MS/MS) method for systematic and comprehensive qualitative analysis of chemical components in Baoxinning capsules, and provide a basis for the development of quality control methods, improvement of standards and clinical application of Baoxinning Capsules. METHODS The chemical components in Baoxinning capsules were analyzed by UHPLC-LTQ-ORBITRAP-MS/MS. The accurate molecular weight, reference substance and multistage fragment ion information of the compound were obtained by UHPLC-LTQ-ORBITRAP-MS/MS, combined with relevant literature and cracking law. RESULTS A total of 103 chemical constituents were identified, including terpenoids, saponins, phenolic acids, flavonoids, organic acids, coumarins, phthalides, curcumins and others. CONCLUSION A rapid qualitative analysis method of chemical components in Baoxinning capsules is established,and this study has a certain reference significance for the quality evaluation index and pharmacodynamic material basis of Baoxinning capsule.
杜亚强, 杨欢, 谭春梅, 陈碧莲, 罗镭. 基于UHPLC-LTQ-Orbitrap-MS/MS技术快速分析保心宁胶囊中的化学成分[J]. 中国药学杂志, 2022, 57(21): 1842-1850.
DU Ya-qiang, YANG Huan, TAN Chun-mei, CHEN Bi-lian, LUO Lei. Rapid Analysis of Chemical Components in Baoxinning Capsules Based on UHPLC-LTQ-Orbitrap-MS/MS Technology. Chinese Pharmaceutical Journal, 2022, 57(21): 1842-1850.
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