Abstract：OBJECTIVE To clarify the chemical constituents of Zhengxin Jiangzhi tablets systematically and establish a rapid analysis method for this traditional Chinese medicine preparation.CONCLUSION UHPLC-Q-exactive orbitrap MS was used to analyze the compounds of Zhengxin Jiangzhi tablets. The chromatographic separation was performed on a Thermo AccucoreaQ RP18(2.1 mm×150 mm, 2.6μm) column with gradient elution using acetonitrile(A)-0.1% formic acid-water(B) as the mobile phase. The flow rate was 0.3 mL·min-1. A heated electrospray ion source was used to ionize compounds. The MS and MS2 data was collected in positive and negative ion modes simultaneously. The chemical constituents of Zhengxin Jiangzhi tablets were identified based on accurate MS data, fragmentation characteristics of MS2, published literature, and reference standards.RESULTS A total of 105 chemical constituents from Zhengxin Jiangzhi tablets were identified, including 21 organic acids, 59 flavones(37 flavone O-glycosides, 10 flavone C-glycosides, 12 polymethoxy flavones), 19 quinones(11 anthraquinones and 8 phenanthraquinones), 3 stilbenes, and 3 other types of compounds. 29 compounds were identified by comparison with reference standards, and the herb sources of each compound were assigned.CONCLUSION The chemical constituents of Zhengxin Jiangzhi tablets are characterized systematically, which lays a foundation for the study of its pharmacodynamic material basis, quality control, and action mechanism.
CHEN J F,LEI P,FAN H,et al. A theory for fifty years——retrospect of the origin and development of Lei Zhongyi′s theory of intermingled phlegm and blood stasis in chest discomfort[J]. J Shaanxi Univ Chin Med[J]. J Shaanxi Univ Chin Med(陕西中医药大学学报), 2019, 42(4): 8-11.
[2]
YAN X,ZHANG N,JI Z S,et al. Chemical constituents of Shenkun Yangxue Granules based on UPLC-Q-Orbitrap HRMS [J]. Chin Tradit Herb Drugs(中草药), 2018, 49(18): 4231-4240.
[3]
TANG M,GAO X,GENG T,et al. Identification of chemical constituents in Qiwei Tongbi Oral Liquid by HPLC-Q-TOF-MS/MS [J]. Chin Tradit Herb Drugs(中草药), 2021, 52(8): 2226-2236.
[4]
LI Z P,WANG J L,LIU S J,et al. Quality control for 7 components in Zhengxin Jiangzhi Tablets based on quantitative analysis of multi-components with a single-marker [J]. Northwest Pharm J(西北药学杂志), 2020, 35(4): 510-516.
[5]
CUI X M,JIN Y T,REN H,et al. Content determination of multiple indexes in zhengxin Jiangzhi Tablets and cluster heatmap analysis [J]. J China Pharm(中国药房), 2021, 32(22): 2743-2747.
[6]
LONG H P,LI X,WANG T T,et al. Study on chemical constituents from Xiaoer Fupi Granules based on UPLC-LTQ-Orbitrap-MS [J]. Chin Tradit Herb Drugs(中草药), 2018, 49(23): 5522-5531.
[7]
TU Y,SUN L N,DONG Z Y,et al. Study on chemical constituents differences of salviae miltiorrhizae Radix et Rhizoma from different producing areas based on UPLC-QTOF-MS Technology [J]. J Chin Med Mater(中药材), 2021,44(6): 1337-1342.
[8]
LIU R,WANG R,PEI K,et al. Rapid identification of chemical constituents of pimpinella the llungiana by UHPLC-Q-Orbitrap HRMS [J]. Chin J Exp Tradit Med Form(中国实验方剂学杂志), 2020, 26(5): 145-151.
[9]
WANG Y L,ZHNG C C,ZHNG F,et al. Chemical profiling and tissue distribution study of Jingyin Granules in rats using UHPLC-Q-Exactive Orbitrap HR-MS [J]. China J Chin Mater Med(中国中药杂志), 2020, 45(22): 5537-5554.
[10]
HONG F,YAN Y,ZHAO L,et al. Analysis on ingredients and metabolites of Gegen Decoction absorbed into blood based on UHPLC-Q-TOF-MS [J]. China J Chin Mater Med(中国中药杂志), 2021, 46(22): 5944-5952.
[11]
SUN D M,DONG Y J,XU A L,et al. Rapid analysis on flavonoids in Gegen Zhiju Soft Capsule by UPLC/Q-TOF-MS [J]. Chin Tradit Herb Drugs(中草药), 2015, 46(7): 970-976.
[12]
HUANG M Q,SUN L N,DONG Z Y,et al. Comparative study on the chemical components between integrated processing and traditional processing of Polygonum multiflorum [J]. Chin Tradit Herb Drugs(中草药): 2022,in publishing.
[13]
XIAO G L,JIANG J Y,LI S M,et al. Analysis of chemical constituents in microctis folium by UPLC-Q-TOF-MS/MS [J]. Chin J Exp Tradit Med Form(中国实验方剂学杂志), 2021, 27(3): 138-148.
[14]
ZHANG K,XU X,LI T,et al. Chemome profiling of Citri Reticulatae Pericarpium using UHPLC-IT-TOF-MS [J]. China J Chin Mater Med(中国中药杂志), 2020, 45(4): 899-909.
[15]
ZHANG D J,LI W,HE Q W,et al. Chemical variation in Aurantii Fructus before and after processing based on UHPLC-Q-TOF-MS [J]. China J Chin Mater Med(中国中药杂志), 2016, 41(11): 2070-2080.
[16]
AN Y,YANG J H,WANG C C,et al. Analysis of chemical constitutents in Cassiae Semen by UHPLC/Q-TOF-MS [J]. Chin Tradit Pat Med(中成药), 2017, 39(4): 776-781.
[17]
DONG H J,CHEN X H,ZENG R. Rapid analysis on chemical constituents in roots of Rheum pumilum by UPLC coupled with hybrid quadrupole-orbit trap MS [J]. Chin Tradit Herb Drugs(中草药), 2016, 47(14): 2428-2435.
[18]
LUO Y Y,LIU J X,LIU X H,et al. Difference of chemical compositions in polygoni multifori Radix from different habitats by UPLC-Triple TOF MS/MS [J]. J Chin Mass Spectrom Soc(质谱学报), 2017, 38(6): 678-689.
[19]
LAI L C,LIN Y Y,CHEN F L,et al. Analysis of main active components in Desmodii Styraciflii Herba by HPLC-Q-TOF-MS and HPLC-DAD [J]. Chin Tradit Herb Drugs(中草药), 2016, 47(20): 3578-3585.
[20]
SUN Z,ZHOU L,LI Z L,et al. The chemical constituents study of Naoxintong capsules based on UHPLC-Q-Orbitrap HRMS [J]. Chin Pharm J(中国药学杂志), 2018, 53(19): 1687-1695.
[21]
WANG Z,ZHONG L Y,JIE Y,et al. Research progress on Polygoni Multiflori Radix based on “raw and cooked with different uses” and prediction analysis on quality marker(Q-Marker)[J]. Chin Tradit Herb Drugs(中草药), 2022, 53(3): 882-897.
[22]
WANG X,WANG L,ZHANG B,et al. Research progress on herbaceous, chemical constituents and pharmacological effects of different medicinal parts of Sophora japonica [J]. Chin Tradit Herb Drugs(中草药), 2018, 49(18): 4461-4467.
[23]
CHEN Y,WEN J Y,XIE X F,et al. Research progress on chemical constituents and pharmacological effects of Gegen [J]. Pharm Clin Chin Mater Med(中药与临床), 2021, 12(1): 53-60.
[24]
FENG K R,LI W X,WANG X Y,et al. Chemical components and pharmacological action for Salviae Miltiorrhizae Radix et Rhizoma and predictive analysis on quality markers [J]. Chin Tradit Herb Drugs(中草药), 2022, 53(2): 609-618.
[25]
LI F,ZHANG T,HE Y,et al. Inflammation inhibition and gut microbiota regulation by TSG to combat atherosclerosis in ApoE/ mice [J]. J Ethnopharmacol, 2019, 247. Doi:10.1016/j.jep.2019.112232.
2022, Vol. 57 
