Reliability of Ultrafiltration Centrifugation in the Evaluation of Entrapment Efficiency: A Case Study of Panax notoginseng Saponins Chitosan Nanoparticles
ZHANG Ruia, WEN Qin-nanb, GUAN Zhi-yub, SHI Minc, XU Pengd
a. Key Laboratory of Modern Preparation of TCM; b.College of Pharmacy; c.School of Basic Medicine; d.Laboratory Animal Center, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China
Abstract：OBJECTIVE To establish a method for determining the entrapment efficiency of Panax notoginseng saponin chitosan nanoparticles(PNS-NPs), and to explore the causes and solutions that may lead to wrong results. METHODS The aggregation state of saponin molecules was destroyed by adding penetration enhancers, and then the encapsulated drugs and free drugs in PNS-NPs were separated by ultrafiltration centrifugation, to avoid nonspecific interception of free drugs by ultrafiltration membrane. In this experiment, the type of penetration enhancer was selected, the recovery and repeatability of ultrafiltration centrifugation method were verified, and the entrapment efficiency of multiple batches of PNS NPs was measured. RESULTS The method can effectively separate encapsulated drugs from free drugs in chitosan nanoparticles, and the recovery and repeatability meet the requirements of determination.. CONCLUSION The separation method can be applied to the determination of encapsulation efficiency of nano preparations prone to intermolecular association drugs, so as to avoid misleading experimental results.
张锐,温沁楠,关志宇,施旻,徐彭. 以三七总皂苷壳聚糖纳米粒为例探讨超滤离心法在包封率评价中的可靠性[J]. 中国药学杂志, 2022, 57(16): 1367-1373.
ZHANG Rui, WEN Qin-nan, GUAN Zhi-yu, SHI Min, XU Peng. Reliability of Ultrafiltration Centrifugation in the Evaluation of Entrapment Efficiency: A Case Study of Panax notoginseng Saponins Chitosan Nanoparticles. Chinese Pharmaceutical Journal, 2022, 57(16): 1367-1373.
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2022, Vol. 57 
