Liposomal Doxorubicin Loaded PLGA-PEG-PLGA for the Treatment of Osteosarcoma
LIANG Guo-hui1, MA Kun1, XIE Yan1, FAN Yong-chun2, ZHAO Cheng-lei2*
1. Luoyang Orthopedic-Traumatological Hospital of Henan Province, Luoyang 471002, China; 2. Jiangsu Province Academy of Traditional Chinese medicine, Nanjing 210028, China
Abstract：OBJECTIVE To prepare the hybrid system of liposomal doxorubicin (DOX-Lip) loaded thermogel (DOX-Lip-Gel) and investigate the in vitro properties and antitumor effect. METHODS Doxorubicin liposomes were prepared by hydration membrane method. Their particle size distribution and entrapment efficiency were investigated. And the in vitro release, viscosity measurement and the anti-tumor efficiency of DOX-Lip-Gel were also investigated. RESULTS The DOX-Lip was prepared with the particle size of (89.3±4.7) nm and the drug entrapment efficiency of (85.3±2.6)%, the liposomes loaded hydrogel was in a sol state at 25 ℃ and converted into the gel state at 37 ℃ with the phase transition temperature of 30 ℃ and could degrade gradually during the time in vivo. The DOX release out of DOX-Lip-Gel could be in a sustained manner up to 200 h with no burst release. An orthotopic osteosarcoma model was adopted and the results revealed that DOX-Lip-Gel had the better antitumor efficiency. CONCLUSION DOX-Lip-Gel could significantly inhibit the growth of osteosarcoma in mice orthotopic model.
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2022, Vol. 57 
